By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Data detailed on DNA Research have been presented. According to news reporting originating in London, United Kingdom, by NewsRx journalists, research stated, "Gold nanoparticles (Au NPs) are used in many fields, including biomedical applications; however, no conclusive information on their potential cytotoxicity and genotoxicity mechanisms is available. For this reason, experiments in human primary lymphocytes and murine macrophages (Raw264.7) were performed exposing cells to spherical citrate-capped Au NPs with two different nominal diameters (5 nm and 15 nm)."
The news reporters obtained a quote from the research from Natural History Museum, "The proliferative activity, mitotic, apoptotic, and necrotic markers, as well as chromosomal damage were assessed by the cytokinesis-block micronucleus cytome assay. Fluorescence in situ hybridization with human and murine pancentromeric probes was applied to distinguish between clastogenic and aneuploidogenic effects. Our results indicate that 5 nm and 15 nm Au NPs are able to inhibit cell proliferation by apoptosis and to induce chromosomal damage, in particular chromosome mis-segregation. DNA strand breaks were detected by comet assay, and the modified protocol using endonuclease-III and formamidopyrimidine-DNA glycosylase restriction enzymes showed that pyrimidines and purines were oxidatively damaged by Au NPs. Moreover, we show a size-independent correlation between the cytotoxicity of Au NPs and their tested mass concentration or absolute number, and genotoxic effects which were more severe for Au NP 15 nm compared to Au NP 5 nm."
According to the news reporters, the research concluded: "Results indicate that apoptosis, aneuploidy, and DNA oxidation play a pivotal role in the cytotoxicity and genotoxicity exerted by Au NPs in our cell models."
For more information on this research see: Aneuploidogenic effects and DNA oxidation induced in vitro by differently sized gold nanoparticles. International Journal of Nanomedicine, 2014;9():2191-2204. International Journal of Nanomedicine can be contacted at: Dove Medical Press Ltd, PO Box 300-008, Albany, Auckland 0752, New Zealand (see also DNA Research).
Our news correspondents report that additional information may be obtained by contacting S. Di Bucchianico, Nat Hist Museum, London SW7 5BD, United Kingdom. Additional authors for this research include M.R. Fabbrizi, S. Cirillo, C. Uboldi, D. Gilliland, E. Valsami-Jones and L. Migliore.
Keywords for this news article include: London, Europe, DNA Research, Nanoparticle, United Kingdom, Nanotechnology, Emerging Technologies
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