News Column

Recent Data from H. Neubacher and Co-Authors Highlight Findings in Antibiotics

June 2, 2014

By a News Reporter-Staff News Editor at Anti-Infectives Week -- Investigators discuss new findings in Drugs and Therapies. According to news reporting originating from Gottingen, Germany, by NewsRx correspondents, research stated, "Screening tools to study antimicrobial peptides (AMPs) with the aim to optimize therapeutic delivery vectors require automated and parallelized sampling based on chip technology. Here, we present the development of a chip-based assay that allows for the investigation of the action of AMPs on planar lipid membranes in a time-resolved manner by fluorescence readout."

Our news editors obtained a quote from the research, "Anodic aluminum oxide (AAO) composed of cylindrical pores with a diameter of 70 nm and a thickness of up to 10 mu m was used as a support to generate pore-spanning lipid bilayers from giant unilamellar vesicle spreading, which resulted in large continuous membrane patches sealing the pores. Because AAO is optically transparent, fluid single lipid bilayers and the underlying pore cavities can be readily observed by three-dimensional confocal laser scanning microscopy (CLSM). To assay the permeabilizing activity of the AMPs, the translocation of the water-soluble dyes into the AAO cavities and the fluorescence of the sulforhodamine 101 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanol-l-amine triethylammonium salt (Texas Red DHPE)-labeled lipid membrane were observed by CLSM in a time-resolved manner as a function of the AMP concentration. The effect of two different AMPs, magainin-2 and melittin, was investigated, showing that the concentrations required for membrane permeabilization and the kinetics of the dye entrance differ significantly. Our results are discussed in light of the proposed permeabilization models of the two AMPs."

According to the news editors, the research concluded: "The presented data demonstrate the potential of this setup for the development of an on-chip screening platform for AMPs."

For more information on this research see: Permeabilization Assay for Antimicrobial Peptides Based on Pore-Spanning Lipid Membranes on Nanoporous Alumina. Langmuir, 2014;30(16):4767-4774. Langmuir can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; Langmuir -

The news editors report that additional information may be obtained by contacting H. Neubacher, Univ Gofttingen, Inst Organ & Biomol Chem, D-37077 Gottingen, Germany. Additional authors for this research include I. Mey, C. Carnarius, T.D. Lazzara and C. Steinem (see also Drugs and Therapies).

Keywords for this news article include: Antibiotics, Antimicrobials, Europe, Germany, Peptides, Proteins, Gottingen, Nanoporous, Amino Acids, Nanotechnology, Drugs and Therapies, Emerging Technologies

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Anti-Infectives Week

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