By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Pharmacokinetics. According to news reporting originating in Gainesville, Florida, by NewsRx journalists, research stated, "A highly sensitive and specific LC-MS/MS method for the quantitation of largazole thiol, the active species of the marine-derived preclinical histone deacetylase inhibitor, largazole (prodrug), was developed and validated. Largazole thiol was extracted with ethyl acetate from human or rat plasma along with the internal standard, harmine."
The news reporters obtained a quote from the research from the University of Florida, "Samples were separated on an Onyx Monolithic C18 column by a stepwise gradient elution with 0.1% formic acid in methanol and 0.1% aqueous formic acid employing multiple reaction monitoring (MRM) detection. Linear calibration curves were obtained in the range of 12.5-400 ng/mL with 200 L of human plasma. The overall intra-day precision was from 3.87% to 12.6%, and the inter-day precision was from 7.12% to 9.8%. The accuracy at low, medium and high concentrations ranged from 101.55% to 105.84%. Plasma protein bindings of largazole thiol in human and rat plasma as determined by an ultrafiltration method were 90.13% and 77.14%, respectively. Plasma drug concentrations were measured by this LC-MS/MS method. The pharmacokinetics of largazole thiol in rats was studied following i.v. administration at 10 mg/kg and found to follow a two-compartment model. Largazole thiol was rapidly eliminated from systemic circulation within 2 h."
According to the news reporters, the research concluded: "The established LC-MS/MS method is suitable for the analysis of largazole thiol in human plasma, as well."
For more information on this research see: Largazole pharmacokinetics in rats by LC-MS/MS. Marine Drugs, 2014;12(3):1623-40 (see also Pharmacokinetics).
Our news correspondents report that additional information may be obtained by contacting M. Yu, Dept. of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL 32610, United States. Additional authors for this research include L.A. Salvador, S.K. Sy, Y. Tang, R.S. Singh, Q.Y. Chen, Y. Liu, J. Hong, H. Derendorf and H. Luesch.
Keywords for this news article include: Pharmaceuticals, Drugs, Florida, Therapy, Gainesville, United States, Pharmacokinetics, North and Central America.
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