By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Nanoparticles. According to news reporting out of Changchun, People's Republic of China, by NewsRx editors, research stated, "The purpose of this study was to investigate the permeability of exendin-4-loaded chitosan nanoparticles using the Madin Darby canine kidney (MDCK) cell monolayer as an in vitro model and the rat intestine as an ex vivo model of the human intestinal barrier. A series of formulations of sodium tripolyphosphate (TIP) and chitosan with different molecular weights and degrees of deacetylation was evaluated."
Our news journalists obtained a quote from the research from Jilin University, "The formulation consisting of 0.1% TIP and 0.2% chitosan (400 kDa, 95% degree of deacetylation), which gave optimized monodispersed particle size (303.1 +/- 10.36nm), zeta potential (18.37 +/- 1.15mV) and encapsulation efficiency (38.0 +/- 2.6%), was used for further analysis. After determining their biocompatibility, the transport potential of drug-loaded chitosan nanoparticles was evaluated and compared with free exendin-4 using both MDCK cell monolayers and different rat intestinal segments. Mechanisms underlying enhanced transport of exendin-4 in the cell model were also explored. Compared with free exendin-4, the absorption of optimized chitosan nanoparticles was enhanced by 4.7-fold in MDCK cell monolayers and by 2.0-2.78-fold in different rat intestinal segments, with no significant difference between the duodenum, jejunum and ileum. As supported by confocal laser scanning microscopic analysis, the lower enhancement of absorption in the intestine compared to the cell monolayer likely resulted from the chitosan nanoparticle-mediated opening of cellular tight junctions and not through intracellular transport."
According to the news editors, the research concluded: "These findings suggest that the potential application of chitosan nanoparticles as delivery carriers of exendin-4 is limited and may need further modifications."
For more information on this research see: Permeability of Exendin-4-Loaded Chitosan Nanoparticles across MDCK Cell Mono layers and Rat Small Intestine. Biological & Pharmaceutical Bulletin, 2014;37(5):740-747. Biological & Pharmaceutical Bulletin can be contacted at: Pharmaceutical Soc Japan, 2-12-15 Shibuya, Shibuya-Ku, Tokyo, 150-0002, Japan (see also Nanoparticles).
Our news journalists report that additional information may be obtained by contacting M.S. Wang, Jilin University, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, People's Republic of China. Additional authors for this research include Y. Zhang, B.X. Sun, Y.N. Sun, X. Gong, Y.G. Wu, X.Z. Zhang, W. Kong and Y. Chen.
Keywords for this news article include: Asia, Changchun, Nanotechnology, Emerging Technologies, People's Republic of China
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