News Column

New Findings Reported from Huazhong University of Science and Technology Describe Advances in DNA Vaccines

June 4, 2014

By a News Reporter-Staff News Editor at Vaccine Weekly -- Data detailed on Biotechnology have been presented. According to news reporting originating from Wuhan, People's Republic of China, by NewsRx correspondents, research stated, "To investigate the effects of co-delivering IL-6 expressing plasmid pCI-IL-6 on the immunogenicity of the anti-caries DNA vaccine pCIA-P, which encodes the surface protein antigen PAc of Streptococcus mutans. Plasmid pCI-IL-6 was constructed by inserting the murine IL-6 gene into the pCI vector."

Our news editors obtained a quote from the research from the Huazhong University of Science and Technology, "Expression of IL-6 in vitro was assessed using Western blot analysis. BALB/c mice were intranasally co-immunized with pCIA-P plus pCI-IL-6 on d 0 and 14. Anti-PAc IgG and secretory IgA (sIgA) were assessed by ELISA. Splenocytes from the mice were re-stimulated with the PAc protein, and IFN-gamma and IL-4 production was measured using ELISA. Splenocyte proliferation was analyzed with flow cytometry. Rats were similarly immunized, and dental caries scores were determined using the Keyes method. Marked expression of IL-6 was found in COS-7 cells transfected with pCI-IL-6. In the pCI-IL-6 co-immunized mice, the specific IgG antibodies in serum and sIgA antibodies in saliva were significantly higher than those in the control mice at weeks 4 and 8. Moreover, the secretion of IFN-gamma from splenocytes in response to re-stimulation with PAc protein was significantly higher in the pCI-IL-6 co-immunized mice than that in the control mice, whereas the secretion of IL-4 had no significant difference. The proliferation of splenocytes from the pCI-IL-6 co-immunized mice was significantly higher than that from the mice immunized with pCIA-P and pCI vector. In the rat caries model, the pCI-IL-6 co-immunization rats displayed lower caries scores than the control rats."

According to the news editors, the research concluded: "Intranasal co-delivery of IL-6 gene significantly enhances the immunogenicity of the anti-caries DNA vaccine."

For more information on this research see: Intranasal co-delivery of IL-6 gene enhances the immunogenicity of anti-caries DNA vaccine. Acta Pharmacologica Sinica, 2014;35(5):592-598. Acta Pharmacologica Sinica can be contacted at: Acta Pharmacologica Sinica, 294 Tai-Yuan Rd, Shanghai, 200031, Peoples R China. (Nature Publishing Group -; Acta Pharmacologica Sinica -

The news editors report that additional information may be obtained by contacting L.K. Su, Huazhong University of Science & Technology, Tongji Hosp, Tongji Med College, Center Stomatol, Wuhan 430030, People's Republic of China. Additional authors for this research include F. Yu, Z.F. Li, C. Zeng, Q.A. Xu and M.W. Fan (see also Biotechnology).

Keywords for this news article include: Asia, Antibodies, Biotechnology, Wuhan, Immunology, DNA Research, DNA Vaccines, Blood Proteins, Immunoglobulins, Synthetic Vaccines, Biological Products, People's Republic of China

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Source: Vaccine Weekly

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