By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Enzymes and Coenzymes. According to news reporting out of Chengdu, People's Republic of China, by NewsRx editors, research stated, "In order to study the structure and expression patterns of uridine diphosphate glucose dehydrogenase (UDPGH) genes in Ipomoea batatas, the transcriptome database of this plant constructed in our lab was first analyzed to screen UDPGH contigs. It was found that there were 23 UDPGH contigs of different sizes in the transcriptome database."
Our news journalists obtained a quote from the research from Sichuan University, "Primers were designed to amplify the coding regions of UDPGH, and five UDPGH-coding genes (named IbUDPGH1-IbUDPGH5) were cloned and sequenced. Open reading frames of all the UDPGH were 1443 bp in length, and their identity was more than 97 and 99% at the nucleotide and protein level, respectively. Homology comparison among different plant UDPGH showed that the identity ranged from 73 to 95% at the nucleotide level and from 84 to 95% at the protein level. The results of digital gene expression profile analysis (DGE) displayed that IbUDPGH1 had the highest expression in the tuberous roots, lower in the young and mature leaves, and the lowest in stems and fibrous roots, while IbUDPGH2 and IbUDPGH5 had the highest transcript level in stems, lower in roots, and very low in leaves. The rest genes were expressed at a low level in different tissues. Semi-quantitative RT-PCR results were similar to above data from the DGE."
According to the news editors, the research concluded: "These results imply that the high expression of UDPGH might make large contribution to the accumulation of cell wall polysaccharides in sweet potato stems and roots."
For more information on this research see: Cloning and characterization of uridine diphosphate glucose dehydrogenase gene from Ipomoea batatas. Russian Journal of Plant Physiology, 2014;61(3):298-308. Russian Journal of Plant Physiology can be contacted at: Maik Nauka, Interperiodica, Springer, 233 Spring St, New York, NY 10013-1578, USA. (Pleiades Publishing - www.maik.ru)
Our news journalists report that additional information may be obtained by contacting X.J. Lai, Sichuan University, Coll Life Sci, Key Lab Resource Biol & Ecoenvironm, Sichuan Key Lab Mol Biol & BiotechnolMinist Educ, Chengdu 610064, People's Republic of China. Additional authors for this research include Y.H. Gu, X. Tao, Y.Z. Zhang and H.Y. Wang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Anions, Chengdu, Electrolytes, Polyphosphates, Oxidoreductases, Phosphoric Acids, Phosphorus Compounds, Enzymes and Coenzymes, People's Republic of China, Carbohydrate Dehydrogenases, Uridine Diphosphate Glucose Dehydrogenase
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