News Column

New Cancer Gene Therapy Findings Reported from University of Fudan

June 2, 2014



By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Investigators discuss new findings in Biotechnology. According to news originating from Shanghai, People's Republic of China, by NewsRx correspondents, research stated, "Tumor cell invasion into the surrounding matrix has been well documented as an early event of metastasis occurrence. However, the dynamic expression patterns of proteins during early invasion of hepatocellular carcinoma (HCC) are largely unknown."

Our news journalists obtained a quote from the research from the University of Fudan, "Using a three-dimensional HCC invasion culture model established previously, we investigated the dynamic expression patterns of identified proteins during early invasion of HCC. Highly metastatic MHCC97H cells and a liver tissue fragment were long-term co-cultured in a rotating wall vessel (RWV) bioreactor to simulate different pathological states of HCC invasion. The established spherical co-cultures were collected on days 0, 5, 10, and 15 for dynamic expression pattern analysis. Significantly different proteins among spheroids at different time points were screened and identified using quantitative proteomics of iTRAQ labeling coupled with LC-MS/MS. Dynamic expression patterns of differential proteins were further categorized by K-means clustering. The expression modes of several differentially expressed proteins were confirmed by Western blot and qRT-PCR. Time course analysis of invasion/metastasis gene expressions (MMP2, MMP7, MMP9, CD44, SPP1, CXCR4, CXCL12, and CDH1) showed remarkable, dynamic alterations during the invasion process of HCC. A total of 1,028 proteins were identified in spherical co-cultures collected at different time points by quantitative proteomics. Among these proteins, 529 common differential proteins related to HCC invasion were clustered into 25 types of expression patterns. Some proteins displayed significant dynamic alterations during the early invasion process of HCC, such as upregulation at the early invasion stage and downregulation at the late invasion stage (e.g., MAPRE1, PHB2, cathepsin D, etc.) or continuous upregulation during the entire invasion process (e.g., vitronectin, Met, clusterin, ICAM1, GSN, etc.)."

According to the news editors, the research concluded: "Dynamic expression patterns of candidate proteins during the early invasion process of HCC facilitate the discovery of new molecular targets for early intervention to prevent HCC invasion and metastasis."

For more information on this research see: Dynamic expression patterns of differential proteins during early invasion of hepatocellular carcinoma. Plos One, 2014;9(3):e88543. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)

The news correspondents report that additional information may be obtained from R.X. Chen, Liver Cancer Institute, Zhongshan Hospital, Fudan University & Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, People's Republic of China. Additional authors for this research include H.Y. Song, Y.Y. Dong, C. Hu, Q.D. Zheng, T.C. Xue, X.H. Liu, Y. Zhang, J. Chen, Z.G. Ren, Y.K. Liu and J.F Cui (see also Biotechnology).

Keywords for this news article include: Asia, Biotechnology, Shanghai, Oncology, Peptides, Proteins, Amino Acids, Cancer Gene Therapy, Hepatocellular Carcinoma, People's Republic of China.

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Source: Cancer Gene Therapy Week