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Findings from S. Migaw and Co-Authors Provide New Insights into Pore Forming Cytotoxic Proteins

June 3, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Membrane Proteins have been published. According to news reporting originating from Sousse, Tunisia, by NewsRx correspondents, research stated, "Nine lactic acid bacteria strains showing bacteriocin-like activity were isolated from various fresh fish viscera. The following species were identified based on 16S rDNA sequences: Enterococcus durans (7 isolates), Lactococcus lactis (1) and Enterococcus faecium (1)."

Our news editors obtained a quote from the research, "These strains were active against Listeria innocua and other LAB. Random amplified polymorphic DNA analyses showed four major patterns for the E. durans species. PCR analyses revealed a nisin gene in the genome of the Lc. lactis strain. Genes coding enterocins A, B and P were found in the genome of the E. faecium isolate. Enterocins A and B genes were also present in the genome of E. durans GM19. Hence, this is the first report describing E. durans strains producing enterocins A and B. Electrospray ionization mass spectrometry revealed that the purified bacteriocin produced by the E. durans GMT18 strain had an exact molecular mass of 6,316.89 Da. This bacteriocin was designated as durancin GMT18. Edman sequencing failed to proceed; suggesting that durancin GTM18 may contain terminal lanthionine residues. Overall, the results obtained revealed the presence of a variety of enterococci in Mediterranean fish viscera, as evidenced by their genetic profiles and abilities to produce different bacteriocins."

According to the news editors, the research concluded: "These strains could be useful for food biopreservation or as probiotics."

For more information on this research see: Diversity of bacteriocinogenic lactic acid bacteria isolated from Mediterranean fish viscera. World Journal of Microbiology & Biotechnology, 2014;30(4):1207-17 (see also Membrane Proteins).

The news editors report that additional information may be obtained by contacting S. Migaw, Departement de Biochimie, Faculte de Medecine Ibn El Jazzar, Unite de Recherche U12-ES03, 4002, Sousse, Tunisia. Additional authors for this research include T. Ghrairi, Y. Belguesmia, Y. Choiset, J.M. Berjeaud, J.M. Chobert, K. Hani and T. Haertle.

Keywords for this news article include: Sousse, Africa, Tunisia, Bacteriocins, Membrane Proteins, Bacterial Proteins, Pore Forming Cytotoxic Proteins.

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Source: Life Science Weekly

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