By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Medical Imaging. According to news reporting out of Pohang, South Korea, by NewsRx editors, research stated, "To evaluate the effectiveness of small interfering RNA (siRNA) targeting matrix metalloproteinase 9 (MMP-9) in suppressing granulation tissue formation caused by bare metallic stent placement in a rat urethral model. All experiments were approved by the committee of animal research."
Our news journalists obtained a quote from the research from the Pohang University of Science and Technology, "In 20 Sprague-Dawley male rats (weight range, 300-350 g), a self-expanding metallic bare stent was inserted in the urethra with fluoroscopic guidance. One group of 10 rats (group A) was treated with MMP-9 siRNA/bioreducible branched polyethylenimine-disulfide cross-linked-indocyanine green (bioreducible BPEI-SS-ICG), while the other group of 10 rats (group B) received control siRNA/bioreducible BPEI-SS-ICG treatment. All rats were sacrificed at 4 weeks. The therapeutic effectiveness of the MMP-9 siRNA/bioreducible BPEI-SS-ICG complex was assessed by comparing the two results of retrograde urethrography, histologic examination, and quantification of MMP-9 by using zymography and Western blot analysis between the two groups. The Mann-Whitney U test was used to evaluate differences. Stent placement was successful in all rats without a single case of migration at follow-up. Retrograde urethrography performed 4 weeks after stent placement demonstrated significantly larger luminal diameters of the urethra within the stents in group A compared with those in group B (P = .011). Histologic analysis revealed that the mean percentage of granulation tissue area (P < .001), mean number of epithelial layers (P < .001), and mean thickness of submucosal fibrosis (P < .001) were significantly decreased in group A compared with group B. Meanwhile, the mean density of inflammatory cell infiltration did not significantly differ between the two groups (P = .184). Quantitative analysis disclosed MMP-9 levels to be lower in group A relative to group B, indicating positive inhibition of MMP-9 by MMP-9 siRNA/bioreducible BPEI-SS-ICG."
According to the news editors, the research concluded: "MMP-9 siRNA/bioreducible BPEI-SS-ICG is effective for inhibiting granulation tissue formation after bare metallic stent placement in a rat urethral model."
For more information on this research see: Bioreducible Polymer-delivered siRNA Targeting MMP-9: Suppression of Granulation Tissue Formation after Bare Metallic Stent Placement in a Rat Urethral Model. Radiology, 2014;271(1):87-95. Radiology can be contacted at: Radiological Soc North America, 820 Jorie Blvd, Oak Brook, IL 60523, USA. (Hindawi Publishing - www.hindawi.com; Radiology - www.hindawi.com/journals/rrp/)
Our news journalists report that additional information may be obtained by contacting J.H. Park, Pohang University of Science & Technology, Dept. of Chem, Pohang, South Korea. Additional authors for this research include J.H. Kim, E.Y. Kim, J. Kim, H.Y. Song, W.J. Kim, D. Lee, J. Park and S. Kim (see also Medical Imaging).
Keywords for this news article include: Asia, Pohang, South Korea, Medical Imaging
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