News Column

Data on Life Science Research Described by Researchers at University of Copenhagen

June 3, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news reporting from Copenhagen, Denmark, by NewsRx journalists, research stated, "Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of enzymes available for this purpose."

The news correspondents obtained a quote from the research from the University of Copenhagen, "In the present study, we demonstrate the utility of the divalent uranyl ion in a new procedure for protein purification and tag removal. By employment of a GFP (green florescence protein) recombinant protein we show that uranyl binding to a phosphorylated C-terminal tag enables target protein purification from an E. coli extract by immobilized uranyl affinity chromatography. Subsequently, the tag can be efficiently removed by UV-irradiation assisted uranyl photocleavage."

According to the news reporters, the research concluded: "We therefore suggest that the divalent uranyl ion (UO22+) may provide a dual function in protein purification and subsequent C-terminal tag removal procedures."

For more information on this research see: A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removal. Plos One, 2014;9(3):e91138. (Public Library of Science -; Plos One -

Our news journalists report that additional information may be obtained by contacting Q. Zhang, Dept. of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. Additional authors for this research include T.J. Jorgensen, P.E. Nielsen and N.E Mollegaard (see also Life Science Research).

Keywords for this news article include: Europe, Denmark, Copenhagen, Life Science Research.

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Source: Life Science Weekly

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