News Column

Studies from University of Kentucky Provide New Data on Biochemistry

May 27, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Biochemistry. According to news reporting originating in Lexington, Kentucky, by NewsRx journalists, research stated, "The emerging field of RNA nanotechnology necessitates creation of functional RNA nanoparticles but has been limited by particle instability. It has been shown that the three-way junction of bacteriophage phi29 motor pRNA has unusual stability and can self-assemble from three fragments with high efficiency."

The news reporters obtained a quote from the research from the University of Kentucky, "It is generally believed that RNA and DNA folding is energy landscape-dependent, and the folding of RNA is driven by enthalpy. Here we examine the thermodynamic characteristics of the 3WJ components as 2'-fluoro RNA, DNA, and RNA. It was seen that the three fragments existed either in 3WJ complex or as monomers, with the intermediate of dimers almost undetectable. It seems that the three fragments can lead to the formation of the 3WJ complex efficiently within a rapid time. A low dissociation constant (apparent K-D) of 11.4 nM was determined for RNA, inclusion of 2'-F pyrimidines strengthened the K-D to 4.5 nM, and substitution of DNA weakened it to 47.7 nM. The Delta G(37)degrees, were -36, -28, and -15 kcal/mol for 3WJ(2'-F), 3WJ(RNA), and 3WJ(DNA), respectively."

According to the news reporters, the research concluded: "It is found that the formation of the three-component complex was governed by entropy, instead of enthalpy, as usually found in RNA complexes."

For more information on this research see: Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments. Biochemistry, 2014;53(14):2221-2231. Biochemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biochemistry - www.pubs.acs.org/journal/bichaw)

Our news correspondents report that additional information may be obtained by contacting D.W. Binzel, University of Kentucky, Coll Pharm, Dept. of Pharmaceut Sci, Lexington, KY 40536, United States. Additional authors for this research include E.F. Khisamutdinov and P.X. Guo (see also Biochemistry).

Keywords for this news article include: Kentucky, Lexington, Biochemistry, DNA Research, United States, North and Central America

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Source: Life Science Weekly