By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Viral Genes. According to news reporting out of Halle, Germany, by NewsRx editors, research stated, "The twin-arginine translocation (Tat) system serves to transport folded proteins across membranes of prokaryotes and plant plastids. In Escherichia coil, a complex consisting of multiple copies of TatB and TatC initiates the transport by binding the signal peptides of the Tat substrates."
Our news journalists obtained a quote from the research from the University of Halle-Wittenberg, "Using blue-native polyacrylamide gel electrophoresis, bands of TatBC-containing complexes can be detected at molecular masses of 440 and 580 kDa. We systematically analyzed the formation of Tat complexes with TatB or TatC variants that carried point mutations at selected positions. Several mutations resulted in specific disassembly patterns and alterations in the 440 kDa:580 kDa complex ratios. The 440 kDa complex contains only TatBC, whereas the 580 kDa complex consists of TatABC. Substrate binding results in a TatBC-Tat substrate complex at similar to 500 kDa and a TatABC-Tat substrate complex at similar to 600 kDa. Only the similar to 600 kDa complex was detected with nonrecombinant substrate levels and thus could be the physiologically most relevant species."
According to the news editors, the research concluded: "The results suggest that some TatA is usually associated with TatBC, regardless of substrate binding."
For more information on this research see: The TatBC Complex of the Tat Protein Translocase in Escherichia coli and Its Transition to the Substrate-Bound TatABC Complex. Biochemistry, 2014;53(14):2344-2354. Biochemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biochemistry - www.pubs.acs.org/journal/bichaw)
Our news journalists report that additional information may be obtained by contacting J. Behrendt, University of Halle Wittenberg, Inst Biol, D-06120 Halle, Germany (see also Viral Genes).
Keywords for this news article include: Halle, Europe, Germany, tat Genes, Viral Genes, Escherichia coli, Genetic Phenomena, Genome Components, Enterobacteriaceae, Genetic Structures, Gram-Negative Bacteria
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