By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Gram-Positive Bacteria have been presented. According to news reporting originating in Seoul, South Korea, by NewsRx journalists, research stated, "Microbial culture extracts are used for natural product screening to find antifungal lead compounds. A microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (AK) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi."
The news reporters obtained a quote from the research from Korea University, "A culture extract of Streptomyces sp. strain KP6107 lysed cells of Fusarium oxysporum f.sp. lycopersici which resulted in high AK activity. The active ingredient N-1 was purified from the culture extract using various chromatographic procedures and identified to be the guanidyl-polyol macrolide antibiotic, niphimycin, which is a potent fungal cell membrane disruptor. Niphimycin showed broad-spectrum antifungal activity against Alternaria mali, Aspergillus oryzae, Colletotrichum coccodes, Colletotrichum gloeosporioides, Cercospora canescens, Cylindrocarpon destructans, F. oxysporum f.sp. cucumerinum, F. oxysporum f.sp. lycopersici, and Rhizoctonia solani at concentrations of 8-64?g?ml(-1). Anthracnose development in pepper plants was completely inhibited by treatment with 50 g?ml(-1) niphimycin, which was as effective as chlorothalonil."
According to the news reporters, the research concluded: "These results show that the AK assay is an efficient and selective tool in screening for cell membrane/wall disruptors of plant pathogenic fungi."
For more information on this research see: Identification of antifungal niphimycin from Streptomyces sp. KP6107 by screening based on adenylate kinase assay. Journal of Basic Microbiology, 2013;53(7):581-9. (Wiley-Blackwell - www.wiley.com/; Journal of Basic Microbiology - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-4028)
Our news correspondents report that additional information may be obtained by contacting H.Y. Kim, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea. Additional authors for this research include J.D. Kim, J.S. Hong, J.H. Ham and B.S Kim (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Seoul, Kinase, South Korea, Actinomycetales, Streptomycetaceae, Enzymes and Coenzymes, Gram Positive Bacteria, Gram-Positive Bacteria, Gram Positive Endospore Forming Rods.
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