By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news reporting originating from Veszprem, Hungary, by NewsRx correspondents, research stated, "The IgG N-glycome provides sufficient complexity and information content to serve as an excellent source for biomarker discovery in mammalian health. Since oligosaccharides play a significant role in many biological processes it is very important to understand their structure."
Our news editors obtained a quote from the research from the University of Pannonia, "The glycosylation is cell type specific as well as highly variable depending on the species producing the IgG. We evaluated the variation of N-linked glycosylation of human, bovine, ovine, equine, canine and feline IgG using three orthogonal glycan separation techniques: hydrophilic interaction liquid chromatography (HILIC)-UPLC, reversed phase (RP)-UPLC and capillary electrophoresis with laser induced fluorescence detection (CE-LIF). The separation of the glycans by these high resolution methods yielded different profiles due to diverse chemistries. However, the % abundance of structures obtained by CE-LIF and HILIC-UPLC were similar, whereas the analysis by RP-UPLC was difficult to compare as the structures were separated by classes of glycans (highly mannosylated, fucosylated, bisected, fucosylated and bisected) resulting in the co-elution of many structures."
According to the news editors, the research concluded: "The IgGs from various species were selected due to the complexity and variation in their N-glycan composition thereby highlighting the complementarity of these separation techniques."
For more information on this research see: Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species. Carbohydrate Research, 2014;389():174-185. Carbohydrate Research can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Carbohydrate Research - www.elsevier.com/wps/product/cws_home/424829)
The news editors report that additional information may be obtained by contacting B. Adamczyk, Univ Pannonia, MTA TKI Translat Glyc Res Grp, Veszprem, Hungary. Additional authors for this research include T. Tharmalingam-Jaikaran, M. Schomberg, A. Szekrenyes, R.M. Kelly, N.G. Karlsson, A. Guttman and P.M. Rudd (see also Life Science Research).
Keywords for this news article include: Europe, Hungary, Veszprem, Life Science Research
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