News Column

Researchers at Shaheed Beheshti University of Medical Sciences Report New Data on Helicobacter pylori

May 27, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Gram-Negative Bacteria. According to news reporting originating in Tehran, Iran, by NewsRx journalists, research stated, "Manipulation of clinically significant antibodies can effectively improve the processes of diagnosis and treatment. Affinity maturation process has a significant role in improvement of antibodies efficiency."

The news reporters obtained a quote from the research from the Shaheed Beheshti University of Medical Sciences, "Error-prone PCR technique is one of the proposed methods for improvement of the affinity of antibodies. In the present research, a method was applied to camel heavy-chain antibody (VHH, nanobody) raised against UreC subunit of urease enzyme from Helicobacter pylori. This VHH was used as a starting molecule to construct a highly diversified phage displayed VHH library. The constructed library of nanobody mutants was subjected to several rounds of panning against UreC antigen. High-affinity mutant was selected. Our VHH (HMR23) showed 1.5-fold higher binding activity than the parental VHH."

According to the news reporters, the research concluded: "In addition, the mutant VHH presented a better performance in inhibition of urease activity at low concentrations retaining its specificity and thermal stability."

For more information on this research see: Functional Mutations in and Characterization of VHH Against Helicobacter pylori Urease. Applied Biochemistry and Biotechnology, 2014;172(6):3079-3091. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)

Our news correspondents report that additional information may be obtained by contacting R. Hoseinpoor, Shahid Beheshti Univ Med Sci, Fac Med, Dept. of Clin Biochem, Tehran, Iran. Additional authors for this research include S.L.M. Gargari, I. Rasooli, M. Rajabibazl and B. Shahi (see also Gram-Negative Bacteria).

Keywords for this news article include: Iran, Asia, Antibodies, Tehran, Immunology, Nanobodies, Blood Proteins, Nanotechnology, Immunoglobulins, Helicobacter pylori, Emerging Technologies, Epsilonproteobacteria, Gram-Negative Bacteria

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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