News Column

Research Data from Tokyo University of Agriculture and Technology Update Understanding of Bacterial Infections

May 30, 2014



By a News Reporter-Staff News Editor at Health & Medicine Week -- New research on Bacterial Infections is the subject of a report. According to news reporting originating from Tokyo, Japan, by NewsRx correspondents, research stated, "A Gram-negative, magnetotactic bacterium, Magnetospirillum magneticum AMB-1 produces nano-sized magnetic particles (BacMPs) in the cytoplasm. Although various applications of genetically engineered BacMPs have been demonstrated, such as immunoassay, ligand-receptor interaction or cell separation, by expressing a target protein on BacMPs, it has been difficult to express disulfide-bonded proteins on BacMPs due to lack of disulfide-bond formation in the cytoplasm."

Our news editors obtained a quote from the research from the Tokyo University of Agriculture and Technology, "Here, we propose a novel dual expression system, called in vitro docking, of a disulfide-bonded protein on BacMPs by directing an immunoglobulin Fc-fused target protein to the periplasm and its docking protein ZZ on BacMPs. By in vitro docking, an scFv-Fc fusion protein was functionally expressed on BacMPs in the dimeric or trimeric form."

According to the news editors, the research concluded: "Our novel disulfide-bonded protein expression system on BacMPs will be useful for efficient screening of potential ligands or drugs, analyzing ligand-receptor interactions or as a magnetic carrier for affinity purification."

For more information on this research see: Functional expression of an scFv on bacterial magnetic particles by in vitro docking. Biochemical and Biophysical Research Communications, 2014;445(1):1-5. (Elsevier - www.elsevier.com; Biochemical and Biophysical Research Communications - www.elsevier.com/wps/product/cws_home/622790)

The news editors report that additional information may be obtained by contacting Y. Sugamata, Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan. Additional authors for this research include T. Tanaka, T. Matsunaga and T. Yoshino (see also Bacterial Infections).

Keywords for this news article include: Asia, Tokyo, Japan, Disulfides, Electrolytes, Inorganic Chemicals, Bacterial Infections and Mycoses.

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Source: Health & Medicine Week