News Column

Reports Outline Aspergillus Study Results from Q.Q. Zhao et al

May 30, 2014

By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators discuss new findings in Aspergillus. According to news reporting originating from Shandong, People's Republic of China, by NewsRx correspondents, research stated, "A beta-galactosidase gene from Aspergillus oryzae was engineered utilizing codon usage optimization to be constitutively and highly expressed in the Pichia pastoris SMD1168H strain in a high-cell-density fermentation."

Our news editors obtained a quote from the research, "After fermentation for 96 h in a 50-L fermentor using glucose and glycerol as combined carbon sources, the recombinant enzyme in the culture supernatant had an activity of 4,239.07 U mL(-1) with o-nitrophenyl-beta-D-galactopyranoside as the substrate, and produced a total of extracellular protein content of 7.267 g L-1 in which the target protein (6.24 g L-1) occupied approximately 86 %. The recombinant beta-galactosidase exhibited an excellent lactose hydrolysis ability."

According to the news editors, the research concluded: "With 1,000 U of the enzyme in 100 mL milk, 92.44 % lactose was degraded within 24 h at 60 degrees C, and the enzyme could also accomplish the hydrolysis at low temperatures of 37, 25, and 10 degrees C. Thus, this engineered strain had significantly higher fermentation level of A. oryzae lactase than that before optimization and the beta-galactosidase may have a good application potential in whey and milk industries."

For more information on this research see: High Level Production of beta-Galactosidase Exhibiting Excellent Milk-Lactose Degradation Ability from Aspergillus oryzae by Codon and Fermentation Optimization. Applied Biochemistry and Biotechnology, 2014;172(6):2787-2799. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer -; Applied Biochemistry and Biotechnology -

The news editors report that additional information may be obtained by contacting Q.Q. Zhao, Shandong Academy Pharmaceut Sci, Jinan 250101, Shandong, People's Republic of China. Additional authors for this research include F. Liu, Z.W. Hou, C. Yuan and X.Q. Zhu (see also Aspergillus).

Keywords for this news article include: Asia, Shandong, Aspergillus, Engineering, beta-Galactosidase, Glycoside Hydrolases, Enzymes and Coenzymes, People's Republic of China

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Source: Health & Medicine Week

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