By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Sphingobacterium is now available. According to news reporting out of Zhoushan, People's Republic of China, by NewsRx editors, research stated, "A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa."
Our news journalists obtained a quote from the research from Zhejiang Ocean University, "The sequences of two peptides of the enzyme were all contained in a GMC family oxidoreductase (EFK55866) by mass spectrometry analysis. The optimal pH of the enzyme was around 6.2. The enzyme was stable within a pH range of 5.0-6.6 and was sensitive to heat. G3DH from S. faecium exhibited extremely broad substrate specificity and well regioselectivity to validoxylamine A. The enzyme was completely inhibited by Hg2Cl2 and partly inhibited by Cu2+, Fe2+, Ca2+, and Cd2+. The apparent K (m) values for D-glucose, sucrose, and validoxylamine were calculated to be 1.1, 1.7, and 2.1 mM, respectively."
According to the news editors, the research concluded: "With this purified enzyme, 3-keto sucrose was prepared at pH 5.0, 30 A degrees C for 10 h with a yield of 28.7 %."
For more information on this research see: Purification and Characterization of the Glucoside 3-Dehydrogenase Produced by a Newly Isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. Applied Biochemistry and Biotechnology, 2014;172(8):3913-3925. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
Our news journalists report that additional information may be obtained by contacting J.F. Zhang, Zhejiang Ocean Univ, Sch Food & Pharm, Zhoushan 316004, People's Republic of China. Additional authors for this research include B. Yang, W.Q. Chen and J. Chen (see also Sphingobacterium).
Keywords for this news article include: Asia, Zhoushan, Bacteroidetes, Dehydrogenase, Sphingobacterium, Enzymes and Coenzymes, Gram-Negative Bacteria, People's Republic of China, Gram-Negative Aerobic Bacteria, Gram-Negative Aerobic Rods and Cocci
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC