News Column

Investigators from National Research Council Target Transmission Electron Microscopy

May 27, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Transmission Electron Microscopy are presented in a new report. According to news reporting from Florence, Italy, by NewsRx journalists, research stated, "Metallic nanoparticles (MeNPs) can be formed in living plants by reduction of the metal ions absorbed as soluble salts. It is very likely that plant metabolism has an important role in MeNP biosynthesis."

The news correspondents obtained a quote from the research from National Research Council, "The in vivo formation of silver nanoparticles (AgNPs) was observed in Brassica juncea, Festuca rubra and Medicago sativa. Plants were grown in Hoagland's solution for 30 days and then exposed for 24 h to a solution of 1,000 ppm AgNO3. In the leaf extracts of control plants, the concentrations of glucose, fructose, ascorbic acid, citric acid and total polyphenols were determined. Total Ag content in plant fractions was determined by inductively coupled plasma atomic emission spectroscopy. Despite the short exposure time, the Ag uptake and translocation to plant leaves was very high, reaching 6,156 and 2,459 mg kg(-1) in B. juncea and F. rubra, respectively. Ultrastructural analysis was performed by transmission electron microscopy (TEM), and AgNPs were detected by TEM X-ray microanalysis. TEM images of plant fractions showed the in vivo formation of AgNPs in the roots, stems and leaves of the plants. In the roots, AgNPs were present in the cortical parenchymal cells, on the cell wall of the xylem vessels and in regions corresponding to the pits. In leaf tissues, AgNPs of different sizes and shapes were located close to the cell wall, as well as in the cytoplasm and within chloroplasts. AgNPs were not observed in the phloem of the three plant species. This is the first report of AgNP synthesis in living plants of F. rubra."

According to the news reporters, the research concluded: "The contents of reducing sugars and antioxidant compounds, proposed as being involved in the biosynthesis of AgNPs, were quite different between the species, thus suggesting that it is unlikely that a single substance is responsible for this process. 92 Biology and other natural sciences; 92Cxx Physiological, cellular and medical topics; 92C80 Plant biology."

For more information on this research see: In vivo synthesis of nanomaterials in plants: location of silver nanoparticles and plant metabolism. Nanoscale Research Letters, 2014;9():1-11. Nanoscale Research Letters can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer -; Nanoscale Research Letters -

Our news journalists report that additional information may be obtained by contacting L. Marchiol, CNR, ICCOM, Center Microscopie Elettron Laura Bonzi, I-50019 Florence, Italy. Additional authors for this research include A. Mattiello, F. Poscic, C. Giordano and R. Musetti (see also Transmission Electron Microscopy).

Keywords for this news article include: Italy, Europe, Florence, Nanomaterial, Nanoparticle, Nanotechnology, Emerging Technologies, Transmission Electron Microscopy

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Source: Life Science Weekly

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