News Column

Investigators at Tokyo Institute of Technology Detail Findings in Ischemia

May 26, 2014

By a News Reporter-Staff News Editor at Pain & Central Nervous System Week -- Investigators publish new report on Ischemia. According to news originating from Kanagawa, Japan, by NewsRx correspondents, research stated, "Developing new tools and technologies to enable recording the dynamic changes of multiple neurochemicals is the essence of better understanding of the molecular basis of brain functions. This study demonstrates a microfluidic chip-based online electrochemical system (OECS) for in vivo continuous and simultaneous monitoring of glucose, lactate, and ascorbate in rat brain."

Our news journalists obtained a quote from the research from the Tokyo Institute of Technology, "To fabricate the, microfluidic chip-based detecting system, a microfluidic chip.. with patterned channel is developed into an electrochemical flow cell by incorporating the chip with three surface-modified indium-tin oxide (ITO) electrodes as working electrodes, a Ag/AgCl wire as reference electrode, and a stainless steel tube as counter electrode. Selective detection of ascorbate is achieved by the use of single-walled carbon nanotubes (SWNTs) to largely facilitate the electrochemical oxidation of ascorbate, while a dehydrogenase-based biosensing mechanism with methylene green (MG) adsorbed onto SWNTs as an electrocatalyst for the oxidation of dihydronicotiamide adenine dinucleotide (NADH) is employed for biosensing of glucose and lactate. To avoid the crosstalk among three sensors, the sensor alignment is carefully designed with the SWNT-modified electrode in the upstream channel and paralleled glucose and lactate biosensors in the downstream channels. With the microfluidic chip-based electrochemical flow cell as the detector, an OECS is successfully established by directly integrating the microfluidic chip-based electrochemical flow cell with in vivo microdialysis. The OECS exhibits a good linear response toward glucose, lactate, and ascorbate with less crosstalk."

According to the news editors, the research concluded: "This property, along with the high stability and selectivity, enables the OECS for continuously monitoring three species in rat brain following brain ischemia."

For more information on this research see: Continuous and Simultaneous Electrochemical Measurements of Glucose, Lactate, and Ascorbate in Rat Brain Following Brain Ischemia. Analytical Chemistry, 2014;86(8):3895-3901. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; Analytical Chemistry -

The news correspondents report that additional information may be obtained from Y.Q. Lin, Tokyo Inst Technol, Dept. of Elect Chem, Interdisciplinary Grad Sch Sci & Engn, Midori Ku, Yokohama, Kanagawa 2268502, Japan. Additional authors for this research include P. Yu, J. Hao, Y.X. Wang, T. Ohsaka and L.Q. Mao (see also Ischemia).

Keywords for this news article include: Asia, Japan, Kanagawa, Chemistry, Brain Diseases, Brain Ischemia, Electrochemical, Vascular Diseases, Cerebrovascular Disorders, Central Nervous System Diseases

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Source: Pain & Central Nervous System Week

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