By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in DNA Research. According to news reporting from Jiangsu, People's Republic of China, by NewsRx journalists, research stated, "Nanomaterials as tracing tags have been widely used in biosensors with high sensitivity and selectivity. In this work, a signal amplification electrochemical aptamer sensing strategy for the detection of protein was designed by combining the hybridization-inducing aggregate of DNA-functionalized silver nanoparticles (AgNPs) and differential pulse stripping voltammetry (DPSV) detection."
The news correspondents obtained a quote from the research from Nanjing University, "The multiprobes containing hybridization DNA and aptamers were anchored onto the silver nanoparticles. The protein assay was prepared through the immobilization of capture aptamer that specifically recognizes platelet-derived growth factor (PDGF-BB) on gold nanoparticles modified screen-printed electrode (SPE) array. After a sandwich-type reaction, two kinds of DNA-modified AgNPs were simultaneously added on the electrode surface for specifically recognizing PDGF-BB and forming the AgNPs aggregate caused by in situ hybridization of DNA. Compared to the signal-labeled tag, the tracing aggregate tags showed a strong electroactivity for signal amplification through stripping detection of silver after preoxidation. By using the hybridization-inducing aggregate as electrochemical readouts, the sensor showed wide linear range and low detection limit. The hybridization-inducing AgNPs aggregate were further used as tracing tags in multiplied proteins assays for PDGF-BB and thrombin by using the SPE array chip as sensing platform. The cross-talk between different aptamer-modified electrodes on the same array was avoided because of the advantage of labeled AgNPs. The array detection was also applied in the logic gate operation."
According to the news reporters, the research concluded: "The proposed method described here is ideal for multianalytes determination in clinical diagnostics with good analytical performance."
For more information on this research see: Disposable electrochemical aptasensor array by using in situ DNA hybridization inducing silver nanoparticles aggregate for signal amplification. Analytical Chemistry, 2014;86(5):2775-83. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting W. Song, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , 22 Hankou Road, Nanjing, Jiangsu 210093, People's Republic of China. Additional authors for this research include H. Li, H. Liang, W. Qiang and D. Xu (see also DNA Research).
Keywords for this news article include: Asia, Jiangsu, Chemistry, DNA Research, Nanoparticle, Nanotechnology, Electrochemical, Emerging Technologies, People's Republic of China.
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