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Findings in the Area of Apoptosis Reported from Indian Institute of Toxicology Research

May 30, 2014

By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Apoptosis. According to news reporting out of Uttar Pradesh, India, by NewsRx editors, research stated, "Zinc oxide nanoparticles (ZnO NPs) induced macrophage cell death and its mechanism remains to be solved. Herein, we report that ZnO NPs induced ROS generation by depleting antioxidant enzymes, increasing lipid peroxidation and protein carbonyl contents in macrophages."

Our news journalists obtained a quote from the research from the Indian Institute of Toxicology Research, "The oxidative stress was induced by the inhibition of Nrf2 transcription factor release. ZnO NPs also activated the cleavage of apoptosis markers like caspases 3, 8 and 9. gamma H2Ax activation and cleavage of poly (ADP-ribose) polymerase (PARP) that are known indicators of genotoxicity were found to be activated by following p53, p21/waf1 signaling. ZnO NPs increased the number of autophagosomes and autophagy marker proteins such as microtubule-associated protein 1 light chain 3-isoform II (MAP-LC3-II) and Beclin 1 after 0.5-24 h of treatment. Phosphorylated Akt, PI3K and mTOR were significantly decreased on ZnO NPs exposure. Moreover, the apoptotic and autophagic cell death could be inhibited on blocking of ROS generation by N-acetylcysteine (NAC) which demonstrated the critical role of ROS in both types of cell death."

According to the news editors, the research concluded: "In addition, inhibition of LC3-II by siRNA-dependent knockdown attenuated the cleavage of caspase 3. This study demonstrates autophagy supports apoptosis on ZnO NPs exposure."

For more information on this research see: Zinc oxide nanoparticles induce apoptosis by enhancement of autophagy via PI3K/Akt/mTOR inhibition. Toxicology Letters, 2014;227(1):29-40. Toxicology Letters can be contacted at: Elsevier Ireland Ltd, Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland. (Elsevier -; Toxicology Letters -

Our news journalists report that additional information may be obtained by contacting R. Roy, Indian Inst Toxicol Res, CSIR, Elect Microscopy Facil, Lucknow 226001, Uttar Pradesh, India. Additional authors for this research include S.K. Singh, K.S. Chauhan, M. Das, A. Tripathi and P.D. Dwivedi (see also Apoptosis).

Keywords for this news article include: Asia, India, Apoptosis, Chemicals, Chemistry, Zinc Oxide, Nanoparticle, Uttar Pradesh, Nanotechnology, Zinc Compounds, Emerging Technologies

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Health & Medicine Week

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