By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Current study results on Biotechnology have been published. According to news reporting out of Suzhou, People's Republic of China, by NewsRx editors, research stated, "To construct specific vectors and evaluate the biological feature of prostate specific antigen (PSA) promoter/enhancer, three fragments harboring PSA enhancer (PSAE) and promoter (PSAP) were amplified using the PCR method. Two PSA promoters, PSA enhancer and pTAL-Luc, were used to construct PSAP1-Luc, PSAP2-Luc, and PSAE-Luc."
Our news journalists obtained a quote from the research from Soochow University, "These recombinant plasmids were co-transfected with a p-galactosidase plasmid into the three cell lines of PC-3, He la, and MCF-7. The expression levels of p-galactosidase luciferase were respectively measured quantitatively. The recombinant plasmids, PSAP1-Luc, PSAP2-Luc, and PSAELuc, were successfully constructed. Prostate cancer cell PC-3 expressed a higher luciferase activity than that of cervical-cancer cell He la and breast cancer cell MCF-7 after being transfected with one of the three plasmids. Among the three plasmids in the three types of cells studied, the highest luciferase level was observed with the PSAP2-Luc transfection. The PSA promoters and enhancer possessed some tissue-specific activity in reporter gene analysis. Among them, the PSA promoter2 containing 620 bp showed the highest expression efficiency. In the three cell lines tested, the highest tissue-specific activity of luciferase over p-galactosidase was observed in the PC-3 cells."
According to the news editors, the research concluded: "These relatively tissue-specific promoters have an impact in the gene therapy of prostate cancer."
For more information on this research see: Construction and In Vitro Evaluation of Tissue-Specific Prostate-Specific Antigen Promoter/Enhancer-Regulated Vectors. Science of Advanced Materials, 2014;6(4):803-807. Science of Advanced Materials can be contacted at: Amer Scientific Publishers, 26650 The Old Rd, Ste 208, Valencia, CA 91381-0751, USA. (American Scientific Publishers - www.aspbs.com/)
Our news journalists report that additional information may be obtained by contacting J.K. Shen, Soochow Univ, Coll Pharmaceut Sci, Lab Mol Med, Suzhou 215123, People's Republic of China. Additional authors for this research include L.H. Wang, W.L. Zhao, C.G. Wei, L. Xiao, Y. Yang and K. Li (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Suzhou, Oncology, Immunology, Kallikreins, Luciferases, Endopeptidases, Oxidoreductases, Serine Proteases, Neoplasm Antigens, Peptide Hydrolases, Cancer Gene Therapy, Luminescent Proteins, Enzymes and Coenzymes, Biological Tumor Markers, Prostate Specific Antigen, Prostate-Specific Antigen
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