News Column

Findings from Northwestern University Feinberg School of Medicine in Proteomics Reported

May 27, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Proteomics is now available. According to news originating from Chicago, Illinois, by NewsRx correspondents, research stated, "Post-assembly functionalization of supramolecular nanostructures has the potential to expand the range of their applications. We report here the use of the chemoselective native chemical ligation (NCL) reaction to functionalize self-assembled peptide amphiphile (PA) nanofibers."

Our news journalists obtained a quote from the research from the Northwestern University Feinberg School of Medicine, "This strategy can be used to incorporate specific bioactivity on the nanofibers, and as a model, we demonstrate functionalization with the RGDS peptide following self-assembly. Incorporation of bioactivity is verified by the observation of characteristic changes in fibroblast morphology following NCL-mediated attachment of the signal to PA nanofibers. The NCL reaction does not alter the PA nanofiber morphology, and biotinylated RGDS peptide was found to be accessible on the nanofiber surface after ligation for binding with streptavidin-conjugated gold nanoparticles. In order to show that this strategy is not limited to short peptides, we utilized NCL to conjugate yellow fluorescent protein and/or cyan fluorescent protein to self-assembled PA nanofibers. Forster resonance energy transfer and fluorescence anisotropy measurements are consistent with the immobilization of the protein on the PA nanofibers. The change in electrophoretic mobility of the protein upon conjugation with PA molecules confirmed the formation of a covalent linkage."

According to the news editors, the research concluded: "NCL-mediated attachment of bioactive peptides and proteins to self-assembled PA nanofibers allows the independent control of self-assembly and bioactivity while retaining the biodegradable peptide structure of the PA molecule and thus can be useful in tailoring design of biomaterials."

For more information on this research see: Post-Assembly Functionalization of Supramolecular Nanostructures with Bioactive Peptides and Fluorescent Proteins by Native Chemical Ligation. Bioconjugate Chemistry, 2014;25(4):707-717. Bioconjugate Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; Bioconjugate Chemistry -

The news correspondents report that additional information may be obtained from S. Khan, Feinberg Sch Med, Dept. of Med, Chicago, IL 60611, United States. Additional authors for this research include S. Sur, P.Y.W. Dankers, R.M.P. da Silva, J. Boekhoven, T.A. Poor and S.I. Stupp (see also Proteomics).

Keywords for this news article include: Chicago, Illinois, Peptides, Proteins, Nanofiber, Proteomics, Amino Acids, United States, Nanostructural, Nanotechnology, Emerging Technologies, North and Central America, Supramolecular Nanostructures

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Source: Life Science Weekly

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