By a News Reporter-Staff News Editor at Cancer Weekly -- Investigators discuss new findings in Connective Tissue Cells. According to news reporting originating in Hiroshima, Japan, by NewsRx journalists, research stated, "A water-soluble complex of fullerene [C-60]:polyethylene glycol (PEG) (1:350 wt/wt) (C-60-PEG), but not PEG alone, was found in the present study by ESR/DMPO spin-trap method to generate hydroxyl radicals 6.5-fold as abundant as the non-irradiation level, when irradiated with visible light (400-600 nm, 140 J/cm(2): 450-fold as intense as in average outdoor), but not to generate without irradiation."
The news reporters obtained a quote from the research from Prefectural University, "At 3 h after irradiation with C-60-PEG, human fibrosarcoma cells HT1080 were obviously degenerated together with diminished microvilli, cell shrinkage and cell fragmentation as observed by SEM and were shown either for increased cytotoxicity by dual stains with calcein-AM and propidium iodide or for nuclear condensation and fragmentation by Hoechst 33342 stain, any of which were, in contrast, scarcely changed in normal human fibroblastic cells DUMS16 derived from the same connective tissue type as HT1080 cells. Under the conditions, the maximum intracellular uptake amount was more abundant for HT1080 cells than for DUMS16 cells, either by immunostain/fluorography using polyclonal antibody against fullerene [C-60], or by HPLC method indicating the 2.4-fold preferential uptake of C-60-PEG into HT1080 cells, suggested to greater phagocytotic ability characteristic of cancer cells, over DUMS16 cells being non-macrophage-like normal cells."
According to the news reporters, the research concluded: "Thus, C-60-PEG is expected as a photosensitizer for photodynamic therapy with scarce side effects to normal cells and preferential reactive oxygen species generation in cancer cells."
For more information on this research see: Photodynamic anti-cancer effects of fullerene [C-60]-PEG complex on fibrosarcomas preferentially over normal fibroblasts in terms of fullerene uptake and cytotoxicity. Molecular and Cellular Biochemistry, 2014;390(1-2):175-184. Molecular and Cellular Biochemistry can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Molecular and Cellular Biochemistry - www.springerlink.com/content/0300-8177/)
Our news correspondents report that additional information may be obtained by contacting R. Asada, Prefectural Univ Hiroshima, Fac Life & Environm Sci, Lab Cell Death Control BioTechnol, Shobara, Hiroshima 7270023, Japan. Additional authors for this research include F. Liao, Y. Saitoh and N. Miwa (see also Connective Tissue Cells).
Keywords for this news article include: Asia, Japan, Cancer, Carbon, Oncology, Hiroshima, Fullerenes, Fibroblasts, Nanotechnology, Emerging Technologies, Connective Tissue Cells
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