News Column

Study Results from Ghent University Update Understanding of Chromatography

May 23, 2014



By a News Reporter-Staff News Editor at Science Letter -- Investigators discuss new findings in Science. According to news reporting from Ghent, Belgium, by NewsRx journalists, research stated, "Offline two dimensional liquid chromatography (LC) x capillary gel electrophoresis (CGE) and LC x (24) multiplexed-CGE methodologies were developed for the separation of oligonucleotides of therapeutic size. Both ion-pair chromatography (IPC) and ion-exchange chromatography (IEC) were studied as methods for the first dimension and single and multiplexed capillary electrophoresis methods in entangled polymer solutions were used for the second dimension separations."

The news correspondents obtained a quote from the research from Ghent University, "Electrokinetic and pressure injection were evaluated for the analysis of the collected LC fractions. The comprehensive separation was optimized with standard mixtures of poly adenosine, thymidine, cytosine and uracil homodeoxyoligonucleotides up to 35 bases long."

According to the news reporters, the research concluded: "Highly orthogonal methodologies and overall peak capacities of 6435 and 6993 for IPC x CGE and IEC x CGE, respectively, were obtained within a few hours analysis time."

For more information on this research see: Combining liquid chromatography with multiplexed capillary gel electrophoresis for offline comprehensive analysis of complex oligonucleotide samples. Journal of Chromatography A, 2014;1336():87-93. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)

Our news journalists report that additional information may be obtained by contacting P.A. Porebski, University of Ghent, Dept. of Organ Chem, Separat Sci Grp, B-9000 Ghent, Belgium (see also Science).

Keywords for this news article include: Ghent, Europe, Belgium, Science

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Source: Science Letter