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Researchers at Guangdong Pharmaceutical University Have Reported New Data on Chromatography

May 23, 2014

By a News Reporter-Staff News Editor at Science Letter -- Current study results on Laboratory Techniques have been published. According to news reporting out of Guangdong, People's Republic of China, by NewsRx editors, research stated, "In this study, we have proposed and developed a novel, environmental-friendly and simple method for separation of nine hydrophilic and hydrophobic components in Danshen using microemulsion liquid chromatography. The proposed method was optimized via the preliminary screening experiment and the experimental design."

Our news journalists obtained a quote from the research from Guangdong Pharmaceutical University, "The following factors were investigated in preliminary screening experiment: pH of mobile phase, column type, the nature of surfactant, the nature of oil phase and additives. In order to simultaneously optimize resolution and analysis time, the chromatographic optimization function (COF) was adopted to evaluate chromatograms. The central composite design (CCD) was used to create the matrix of experiments for mapping the chromatographic response surface. Finally, the COF values were fitted into a second order polynomial model and the response surface methodology (RSM) was employed to find the optimal eluent constituents. The reliability of the established model was confirmed by the good agreement obtained between experimental data and predictive values."

According to the news editors, the research concluded: "Based on the results from the preliminary screening experiment and the CCD optimization, the optimal mobile phase was identified as a solution consisting of 6.68% (w/w) polyoxyethylene lauryl ether (Brij35), 0.84% (w/w) cyclohexane, 6.92% (w/w) n-butanol, 85.56% (w/w) phosphate buffer (pH 6.60) and 8 mM cetyltrimethyl ammonium bromide (CTAB).(."

For more information on this research see: Optimization of liquid chromatographic method for the separation of nine hydrophilic and hydrophobic components in Salviae miltiorrhizae Radix et Rhizoma (Danshen) using microemulsion as eluent. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2014;955():124-133. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Laboratory Techniques).

Our news journalists report that additional information may be obtained by contacting H.Z. Huang, Guangdong Pharmaceut Univ, Sch Pharm, Dept. of Pharmaceut Anal, Guangzhou 510006, Guangdong, People's Republic of China. Additional authors for this research include X.Y. Xuan, L.Y. Xu, J.R. Yang, C.K. Gao and N. Li.

Keywords for this news article include: Asia, Guangdong, Laboratory Techniques, People's Republic of China

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Source: Science Letter

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