News Column

New Findings Reported from N. De Clercq and Co-Authors Describe Advances in Adrenal Cortex Hormones

May 23, 2014

By a News Reporter-Staff News Editor at Science Letter -- Researchers detail new data in Adrenal Cortex Hormones. According to news reporting originating in Marloie, Belgium, by NewsRx journalists, research stated, "Faecal glucocorticoid analysis is a powerful non-invasive tool for the study of the animal endocrine status and stress physiology, which is mainly carried out by immunoassays, characterised by some limitations. In this study, an ultra high-performance liquid chromatography coupled to high resolution Orbitrap mass spectrometry (U-HPLC-HRMS) method was developed to confirm the presence of glucocorticoids in bovine faeces during a long-term stability study."

The news reporters obtained a quote from the research, "Because of the complex nature of faeces, an appropriate extraction and purification procedure was developed. To this extent, a Plackett-Burman experimental design was successfully applied to determine the key conditions for optimal extraction of glucocorticoids from faeces. The targeted analysis, including natural and synthetic glucocorticoids, was successfully validated according to CD 2002/657/EC. Decision limits and detection capabilities for prednisolone, prednisone, methylprednisolone and the metabolites 20 alpha-dihydroprednisolone and 20 beta-dihydroprednisolone ranged, respectively, from 0.15 to 2.95 mu g kg(-1) and from 0.40 to 5.20 mu g kg(-1). Limits of detection and limits of quantification for the natural glucocorticoids dihydrocortisone, cortisol and cortisone ranged, respectively, from 0.55 to 2.10 mu g kg(-1) and from 0.70 to 5.00 mu g kg(-1)."

According to the news reporters, the research concluded: "The stability study of glucocorticoids in faecal matrix demonstrated that lyophilising the faeces, storage at -80 degrees C, and aerobic conditions were optimal for preservation and able to significantly (p < 0.05) limit degradation up to 10 weeks."

For more information on this research see: Development and validation of a high-resolution mass-spectrometry-based method to study the long-term stability of natural and synthetic glucocorticoids in faeces. Journal of Chromatography A, 2014;1336():76-86. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; Journal of Chromatography A -

Our news correspondents report that additional information may be obtained by contacting N. De Clercq, CER Grp, Dept. of Sante, B-6900 Marloie, Belgium. Additional authors for this research include J.V. Bussche, S. Croubels, P. Delahaut and L. Vanhaecke (see also Adrenal Cortex Hormones).

Keywords for this news article include: Europe, Marloie, Belgium, Glucocorticoids, Adrenal Cortex Hormones

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Source: Science Letter

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