By a News Reporter-Staff News Editor at Science Letter -- Current study results on Immunology have been published. According to news reporting from Nanjing, People's Republic of China, by NewsRx journalists, research stated, "A sandwich-type electrochemical immunosensor for the detection of carbohydrate antigen 19-9 (CA 19-9) antigen based on the immobilization of primary antibody (Ab1) on three dimensional ordered macroporous magnetic (3DOMM) electrode, and the direct electrochemistry of horseradish peroxidase (HRP) that was used as both the label of secondary antibody (Ab2) and the blocking reagent. The 3DOMM electrode was fabricated by introducing core-shell Au-SiO2@Fe3O4 nanospheres onto the surface of three dimensional ordered macroporous (3DOM) Au electrode via the application of an external magnet."
The news correspondents obtained a quote from the research from the College of Science, "Au nanoparticles functionalized SBA-15 (Au@SBA-15) was conjugated to the HRP labeled secondary antibody (HRP-Ab2) through the Au-SH or Au-NH3(+) interaction, and HRP was also used as the block reagent. The formation of antigen-antibody complex made the combination of Au@SBA-15 and 3DOMM exhibit remarkable synergistic effects for accelerating direct electron transfer (DET) between HRP and the electrode. Under the optimal conditions, the DET current signal increased proportionally to CA 19-9 concentration in the range of 0.05 to 15.65 U mL(-1) with a detection limit of 0.01 U mL(-1). Moreover, the immunosensor showed high selectivity, good stability, satisfactory reproducibility and regeneration."
According to the news reporters, the research concluded: "Importantly, the developed method was used to assay clinical serum specimens, achieving a good relation with those obtained from the commercialized electrochemiluminescent method."
For more information on this research see: Amperometric carbohydrate antigen 19-9 immunosensor based on three dimensional ordered macroporous magnetic Au film coupling direct electrochemistry of horseradish peroxidase. Analytica Chimica Acta, 2014;815():42-50. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
Our news journalists report that additional information may be obtained by contacting Q. Zhang, College of Sciences, Nanjing Technical University, Nanjing 211816, People's Republic of China. Additional authors for this research include X. Chen, Y. Tang, L. Ge, B. Guo and C. Yao (see also Immunology).
Keywords for this news article include: Asia, Antibodies, Nanjing, Immunology, Blood Proteins, Immunoglobulins, People's Republic of China.
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