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New Findings from National Institute of Advanced Industrial Science and Technology (NIAIST) Update Understanding of Bovine Serum Albumin

May 23, 2014

By a News Reporter-Staff News Editor at Science Letter -- New research on Proteins is the subject of a report. According to news reporting out of Tokyo, Japan, by NewsRx editors, research stated, "This study highlights the ability of arginine to elute bovine serum albumin (BSA) and a monoclonal antibody against interleukin-8 (mAb-IL8) from Capto MMC, which is a multimodal cation exchanger. Arginine provides high recovery of monomeric BSA from Capto MMC chromatography columns at yields similar to NaCI elution, and oligomeric BSA was more readily eluted by arginine than by NaCI."

Our news journalists obtained a quote from the research from the National Institute of Advanced Industrial Science and Technology (NIAIST), "The effectiveness of arginine as an eluent also enabled the separation of monomeric BSA from the oligomeric forms. The purification of mAb-IL8 was successfully achieved using Capto MMC chromatography and arginine as the eluent. The mechanism of the effects of arginine on protein elution was determined by calculating the binding free energy between arginine and Capto MMC using molecular dynamics simulations. The overall affinity of arginine for Capto MMC was associated with electrostatic interactions."

According to the news editors, the research concluded: "However, additional affinities contributed by hydrophobic interaction or hydrogen bonding were also observed to play a role in the interaction between arginine and Capto MMC, which likely results in the characteristic elution by arginine."

For more information on this research see: Interaction of arginine with Capto MMC in multimodal chromatography. Journal of Chromatography A, 2014;1338():58-66. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; Journal of Chromatography A -

Our news journalists report that additional information may be obtained by contacting A. Hirano, Natl Inst Adv Ind Sci & Technol, Computat Biol Res Center, Koto Ku, Tokyo 1350064, Japan. Additional authors for this research include T. Arakawa and T. Kameda (see also Proteins).

Keywords for this news article include: Asia, Tokyo, Japan, Proteins, Bovine Serum Albumin

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Source: Science Letter

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