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Researchers at National Institute for Biotechnology and Genetic Engineering Have Reported New Data on Molecular Biology

May 20, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Life Science Research is the subject of a report. According to news reporting out of Faisalabad, Pakistan, by NewsRx editors, research stated, "Abiotic stresses such as salinity and drought have adverse effects on plants. In the present study, a Na(+)/H(+) antiporter gene homologue (LfNHX1) has been cloned from a local halophyte grass (Leptochloa fusca)."

Our news journalists obtained a quote from the research from National Institute for Biotechnology and Genetic Engineering, "The LfNHX1 cDNA contains an open reading frame of 1,623 bp that encodes a polypeptide chain of 540 amino acid residues. LfNHX1 protein sequence showed high similarity with NHX1 homologs reported from other halophyte plants. Amino acid and nucleotide sequence similarity, protein topology modeling and the presence of conserved functional domains in the LfNHX1 protein sequence classified it as a vacuolar NHX1 homolog. The overexpression of LfNHX1 gene under CaMV35S promoter conferred salt and drought tolerance in tobacco plants. Under drought stress, transgenic plants showed higher relative water contents, photosynthetic rate, stomatal conductance and membrane stability index as compared to wild type plants. More negative value of leaf osmotic potential was also observed in transgenic plants when compared with wild type control plants. Transgenic plants showed better germination and root growth at 2 mg L(-1) Basta herbicide and three levels (100, 200 and 250 mM) of sodium chloride."

According to the news editors, the research concluded: "These results showed that LfNHX1 is a potential candidate gene for enhancing drought and salt tolerance in crops."

For more information on this research see: Cloning and characterization of Na+/H+ antiporter (LfNHX1) gene from a halophyte grass Leptochloa fusca for drought and salt tolerance. Molecular Biology Reports, 2014;41(3):1669-82. Molecular Biology Reports can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer -; Molecular Biology Reports -

Our news journalists report that additional information may be obtained by contacting M. Rauf, Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan. Additional authors for this research include K. Shahzad, R. Ali, M. Ahmad, I. Habib, S. Mansoor, G.A. Berkowitz and N.A Saeed (see also Life Science Research).

Publisher contact information for the journal Molecular Biology Reports is: Springer, 233 Spring Street, New York, NY 10013, USA.

Keywords for this news article include: Asia, Pakistan, Faisalabad, Life Science Research.

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Source: Life Science Weekly

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