By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Dosage Forms. According to news reporting originating from Gujarat, India, by NewsRx correspondents, research stated, "UV spectrophotometric and high performance liquid chromatography (HPLC) methods were developed for simultaneous determination of meropenem (MERM) and sulbactam sodium (SB) in injection. UV spectrophotometric methods were developed using 0.1 N sodium hydroxide as solvent."
Our news editors obtained a quote from the research from the College of Pharmacy, "The Beer's plot for dual wavelength method was linear in the range of 4-24 mu g mL(-1) and 2-12 mu g mL(-1) for MERM and SB, respectively. The percent recoveries were found to be 98.52 +/- 1.23% for MERM and 101.45 +/- 1.1% for SB. Chemometrics assisted UV spectrophotometry was performed using Partial Least Square (PLS) analysis model and Principal Component Regression (PCR) analysis model. The % recoveries of the MERM were found to be 100.61 +/- 0.06% and 101.31 +/- 0.12% using PLS and PCR, respectively. The % recoveries of the SB were found to be 98.29 +/- 0.09% and 97.61 +/- 0.13% using PLS and PCR, respectively. Chromatography was performed on Hypersil BDS C18 column using methanol:acetonitrile:water (10:20:70 v/v/v) as mobile phase. The retention times of MERM and SB were found to be 2.9 min and 2.25 mm, respectively. Developed HPLC method was found to be linear in the range of 50-250 mu g mL(-1) and 25-125 mu g mL(-1) for MERM and SB, respectively. The % recoveries were found to be 98.85 +/- 0.25% and 98.63 +/- 0.34% for MERM and SB, respectively."
According to the news editors, the research concluded: "The developed analytical methods did not show any interference of the excipients when applied to pharmaceutical dosage form."
For more information on this research see: Ultraviolet spectrophotometry (dual wavelength and chemometric) and high performance liquid chromatography for simultaneous estimation of meropenem and sulbactam sodium in pharmaceutical dosage form. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 2014;124():292-299. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England (see also Dosage Forms).
The news editors report that additional information may be obtained by contacting K.R. Marwada, CHARUSAT, Ramaribhai Patel Coll Pharm, Changa, Gujarat, India. Additional authors for this research include J.B. Patel, N.S. Patel, B.D. Patel, D.V. Borkhatariya and A.J. Patel.
Keywords for this news article include: Asia, India, Gujarat, Chemometric, Dosage Forms, Machine Learning, Imaging Technology, Emerging Technologies, High-Performance Liquid Chromatography
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