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Investigators from University of North Carolina Zero in on Capillary Electrophoresis

May 20, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Capillary Electrophoresis. According to news reporting out of Chapel Hill, North Carolina, by NewsRx editors, research stated, "We describe a chemical vapor deposition (CVD) method for the surface modification of glass microfluidic devices designed to perform electrophoretic separations of cationic species. The microfluidic channel surfaces were modified using aminopropyl silane reagents."

Our news journalists obtained a quote from the research from the University of North Carolina, "Coating homogeneity was inferred by precise measurement of the separation efficiency and electroosmotic mobility for multiple microfluidic devices. Devices coated with (3-aminopropyl)di-isopropylethoxysilane (APDIPES) yielded near diffusion-limited separations and exhibited little change in electroosmotic mobility between pH 2.8 and pH 7.5. We further evaluated the temporal stability of both APDIPES and (3-aminopropyl)triethoxysilane (APTES) coatings when stored for a total of 1 week under vacuum at 4 degrees C or filled with pH 2.8 background electrolyte at room temperature, Measurements of electroosmotic flow (EOF) and separation efficiency during this time confirmed that both coatings were stable under both conditions. Microfluidic devices with a 23 cm long, serpentine electrophoretic separation channel and integrated nanoelectrospray ionization emitter were CVD coated with APDIPES and used for capillary electrophoresis (CE)-electrospray ionization (ESI)-mass spectrometry (MS) of peptides and proteins."

According to the news editors, the research concluded: "Peptide separations were fast and highly efficient, yielding theoretical plate counts over 600,000 and a peak capacity of 64 in less than 90 s. Intact protein separations using these devices yielded Gaussian peak profiles with separation efficiencies between 100,000 and 400,000 theoretical plates."

For more information on this research see: Chemical Vapor Deposition of Aminopropyl Si lanes in Microfluidic Channels for Highly Efficient Microchip Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry. Analytical Chemistry, 2014;86(7):3493-3500. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

Our news journalists report that additional information may be obtained by contacting N.G. Batz, University of North Carolina, Carolina Center Genome Sci, Chapel Hill, NC 27599, United States. Additional authors for this research include J.S. Mellors, J.P. Alarie and J.M. Ramsey (see also Capillary Electrophoresis).

Keywords for this news article include: Chapel Hill, United States, North Carolina, Nanotechnology, Emerging Technologies, North and Central America, Capillary Electrophoresis, Chemical Vapor Deposition

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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