News Column

Findings from Seattle Genetics Provides New Data about Sulfur Amino Acids

May 20, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Sulfur Amino Acids are presented in a new report. According to news reporting originating in Bothell, Washington, by NewsRx journalists, research stated, "Analysis of samples containing intact antibody drug conjugates (ADC) using mass spectrometry provides a direct measurement of the drug-load distribution. Once dosed, the drug load distribution changes due to a combination of biological and chemical factors."

The news reporters obtained a quote from the research from Seattle Genetics, "Liquid chromatography mass spectrometry (LC-MS) methods to measure the in vivo drug load distribution have been established for ADCs containing native disulfide bonds (lysine-linked or cysteine-linked). However, because of an IgG reduction step in conjugation processes, using LC-MS to analyze intact cysteine-linked ADCs requires native conditions, thus limiting sensitivity. While this limitation has been overcome at the analytical scale, to date, these methods have not been translated to a smaller scale that is required for animal or clinical doses/sampling. In this manuscript, we describe the development of ADC specific affinity capture reagents for processing in vivo samples and optimization of native LC-MS methods at a microscale."

According to the news reporters, the research concluded: "These methods are then used to detect the changing drug load distribution over time from a set of in vivo samples, representing to our knowledge the first native mass spectra of cysteine-linked ADCs from an in vivo source."

For more information on this research see: Measurement of in Vivo Drug Load Distribution of Cysteine-Linked Antibody-Drug Conjugates Using Microscale Liquid Chromatography Mass Spectrometry. Analytical Chemistry, 2014;86(7):3420-3425. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

Our news correspondents report that additional information may be obtained by contacting S.M. Hengel, Seattle Genet Inc, Bothell, WA 98021, United States. Additional authors for this research include R. Sanderson, J. Valliere-Douglass, N. Nicholas, C. Leiske and S.C. Alley (see also Sulfur Amino Acids).

Keywords for this news article include: Antibodies, Bothell, Cysteine, Washington, Immunology, United States, Blood Proteins, Immunoglobulins, Sulfur Amino Acids, Neutral Amino Acids, Sulfhydryl Compounds, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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