News Column

Study Results from Aarhus University Broaden Understanding of Dioxygenases

May 7, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Enzymes and Coenzymes have been published. According to news originating from Tjele, Denmark, by NewsRx correspondents, research stated, "Lipoxygenases (LOXs) are well-studied enzymes in plants and mammals. However, fungal LOXs are less studied."

Our news journalists obtained a quote from the research from Aarhus University, "In this study, we have compared fungal LOX protein sequences to all known characterized LOXs. For this, a script was written using Shell commands to extract sequences from the NCBI database and to align the sequences obtained using Multiple Sequence Comparison by Log-Expectation. We constructed a phylogenetic tree with the use of Quicktree to visualize the relation of fungal LOXs towards other LOXs. These sequences were analyzed with respect to the signal sequence, C-terminal amino acid, the stereochemistry of the formed oxylipin, and the metal ion cofactor usage. This study shows fungal LOXs are divided into two groups, the Ile- and the Val-groups. The Ile-group has a conserved WRYAK sequence that appears to be characteristic for fungal LOXs and has as a C-terminal amino acid Ile. The Val-group has a highly conserved WL-L/F-AK sequence that is also found in LOXs of plant and animal origin. We found that fungal LOXs with this conserved sequence have a Val at the C-terminus in contrast to other LOXs of fungal origin. Also, these LOXs have signal sequences implying these LOXs will be expressed extracellularly."

According to the news editors, the research concluded: "Our results show that in this group, in addition to the Gaeumannomyces graminis and the Magnaporthe salvinii LOXs, the Aspergillus fumigatus LOX uses manganese as a cofactor."

For more information on this research see: A novel class of fungal lipoxygenases. Applied Microbiology and Biotechnology, 2014;98(3):1261-1270. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer -; Applied Microbiology and Biotechnology -

The news correspondents report that additional information may be obtained from R. Heshof, Aarhus University, Fac Sci & Technol, Dept. of Food Sci, DK-8830 Tjele, Denmark. Additional authors for this research include S. Jylha, T. Haarmann, A. Jorgensen, T. Dalsgaard and L.H. de Graaff (see also Enzymes and Coenzymes).

Keywords for this news article include: Tjele, Europe, Denmark, Dioxygenases, Lipoxygenase, Metalloproteins, Oxidoreductases, Enzymes and Coenzymes, Nonheme Iron Proteins

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Source: Biotech Week

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