By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news originating from Chongqing, People's Republic of China, by NewsRx correspondents, research stated, "We report on a novel electrochemical signal amplification strategy for use in immunoassays. The highly responsive immunoelectrode was constructed in the following way: (1) The surface of a gold electrode was covered with a layer single-walled carbon nanotubes dispersed in chitosane functionalized with L-cysteine; (2) Gold nanoparticles containing protein A and anti-alpha-fetoprotein (anti-AFP) were then covalently attached to the surface via the thiol groups of the chitosane."
Our news journalists obtained a quote from the research from Southwest University, "The electrode is then exposed to the analyte (AFP) which then is bound by the antibody. In the next step, a gold-conjugated secondary antibody is added that was prepared in the following way: (1) Horseradish peroxidase was crosslinked and the resulting spheres were coated with hollow gold nanoparticles (hollow Au-NPs) to give nanospheres of similar to 100 nm in diameter. (2) These were the coated with thionine and, in a last step, with secondary antibody. The use of these materials has several attractive features: The HRP-NPs functionalized with hollow Au-NPs possess a large surface area that can load the large amount of secondary antibody. Thionine (Thi) is highly redox active and improves the intensity of the signal."
According to the news editors, the research concluded: "Carbon nanotubes were used because they possess an excellent electron transfer rate and large surface area."
For more information on this research see: Horseradish peroxidase-loaded nanospheres attached to hollow gold nanoparticles as signal enhancers in an ultrasensitive immunoassay for alpha-fetoprotein. Microchimica Acta, 2014;181(5-6):679-685. Microchimica Acta can be contacted at: Springer Wien, Sachsenplatz 4-6, PO Box 89, A-1201 Wien, Austria. (Springer - www.springer.com; Microchimica Acta - www.springerlink.com/content/0026-3672/)
The news correspondents report that additional information may be obtained from Y. Li, Southwest University, Coll Chem & Chem Engn, Key Lab Luminescence & Real Time Anal, Minist Educ, Chongqing 400715, People's Republic of China. Additional authors for this research include R. Yuan, Y.Q. Chai, Y. Zhuo, H.L. Su and Y.X. Zhang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Nanotube, Chongqing, Nanosphere, Fetal Proteins, Nanotechnology, Oxidoreductases, Gold Nanoparticles, alpha-Fetoproteins, Emerging Technologies, Enzymes and Coenzymes, Horseradish Peroxidase, Biological Tumor Markers, People's Republic of China
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