News Column

Studies from Institute for Molecular Biology Update Current Data on Biotechnology

May 7, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Technology are presented in a new report. According to news originating from Aachen, Germany, by NewsRx correspondents, research stated, "The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media."

Our news journalists obtained a quote from the research from Institute for Molecular Biology, "Tobacco (Nicotiana tabacum L. cv. Petit Havana SRI) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7 mu g/g dry weight (DW) and a maximum of 31.3 mu g/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3 mu g/g DW following deglycosylation."

According to the news editors, the research concluded: "A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag."

For more information on this research see: Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway. Journal of Biotechnology, 2014;176():20-28. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; Journal of Biotechnology -

The news correspondents report that additional information may be obtained from A. Ritala, Fraunhofer Inst Mol Biol & Appl Ecol IME, D-52074 Aachen, Germany. Additional authors for this research include L.M. Dong, N. Imseng, T. Seppanen-Laakso, N. Vasilev, S. van der Krol, H. Rischer, H. Maaheimo, A. Virkki, J. Brandli, S. Schillberg, R. Eibl, H. Bouwmeester and K.M. Oksman-Caldentey (see also Technology).

Keywords for this news article include: Aachen, Europe, Germany, Technology

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Source: Biotech Week

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