News Column

Researchers' Work from University of Delhi Focuses on Peptides and Proteins

May 7, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Peptides and Proteins have been published. According to news originating from New Delhi, India, by NewsRx correspondents, research stated, "Plantaricin gene-specific primers were used to obtain plnE, -F, -J and -K structural gene amplicons from soil metagenome. These amplicons were cloned and expressed in pET32a (+) vector in Escherichia coli BL21 (DE3)."

Our news journalists obtained a quote from the research from the University of Delhi, "PlnE, -F, -J and -K peptides were expressed as His-tagged-fusion proteins and were separated by Ni2+ -chelating affinity chromatography. The peptides were released from the fusion by enterokinase cleavage and separated from the carrier thioredoxin. The cleaved peptides were further analysed for antimicrobial activity and found to be active against Listeria innocua NRRL B33314, Micrococcus luteus MTCC 106 and lactic acid bacteria, such as Enterococcus casseliflavus NRRL B3502, Lactococcus lactis lactis NRRL 1821, Lactobacillus curvatus NRRL B4562 and Lactobacillus plantarum NRRL B4496. E. coli has been successfully exploited as a host for heterologous expression with a significant yield of fused and cleaved peptides in the range of 8-12 and 1-1.5 mg/l of the culture, respectively."

According to the news editors, the research concluded: "Heterologous expression, therefore, can be used to overcome the constraints of low yield often reported from a native strain."

For more information on this research see: Cloning and heterologous expression of plnE, -F, -J and -K genes derived from soil metagenome and purification of active plantaricin peptides. Applied Microbiology and Biotechnology, 2014;98(3):1441-1447. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Applied Microbiology and Biotechnology - www.springerlink.com/content/0175-7598/)

The news correspondents report that additional information may be obtained from G. Pal, University of Delhi, Dept. of Genet, New Delhi 110021, India (see also Peptides and Proteins).

Keywords for this news article include: Asia, India, Cloning, New Delhi, Amino Acids, Peptides and Proteins

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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