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Researchers from University of Dublin Trinity College Describe Findings in Pharmacology

May 7, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Pharmacology have been published. According to news originating from Dublin, Ireland, by NewsRx correspondents, research stated, "Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological agents widely used in oral medicine and dental practice due to their anti-microbial/anti-cavity properties. However, risks associated with the co-exposure of local cells and tissues to these xenobiotics are not clear."

Our news journalists obtained a quote from the research from the University of Dublin Trinity College, "Therefore, we have evaluated the effects of AgNPs and F co-exposure on human gingival fibroblast cells. Human gingival fibroblast cells (CRL-2014) were exposed to AgNPs and/or F at different concentrations for up to 24 hours. Cellular uptake of AgNPs was examined by transmission electron microscopy. Downstream inflammatory effects and oxidative stress were measured by real-time quantitative polymerase chain reaction (PCR) and reactive oxygen species (ROS) generation. Cytotoxicity and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and real-time quantitative PCR and flow cytometry, respectively. Finally, the involvement of mitogen-activated protein kinases (MAPK) was studied using Western blot. We found that AgNPs penetrated the cell membrane and localized inside the mitochondria. Co-incubation experiments resulted in increased oxidative stress, inflammation, and apoptosis. In addition, we found that co-exposure to both xenobiotics phosphorylated MAPK, particularly p42/44 MAPK. A combined exposure of human fibroblasts to AgNPs and F results in increased cellular damage."

According to the news editors, the research concluded: "Further studies are needed in order to evaluate pharmacological and potentially toxicological effects of AgNPs and F on oral health."

For more information on this research see: Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride. International Journal of Nanomedicine, 2014;9():1677-1687. International Journal of Nanomedicine can be contacted at: Dove Medical Press Ltd, PO Box 300-008, Albany, Auckland 0752, New Zealand (see also Pharmacology).

The news correspondents report that additional information may be obtained from I. Inkielewicz-Stepniak, University of Dublin Trinity College, Trinity Biomed Sci Inst, Dublin 2, Ireland. Additional authors for this research include M.J. Santos-Martinez, C. Medina and M.W. Radomski.

Keywords for this news article include: Dublin, Europe, Anions, Ireland, Therapy, Fibroblasts, Nanoparticle, Pharmacology, Nanotechnology, Sodium Fluoride, Sodium Compounds, Hydrofluoric Acid, Emerging Technologies, Connective Tissue Cells

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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