By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biomedicine and Biomedical Engineering. According to news reporting originating from Houston, Texas, by NewsRx correspondents, research stated, "This study investigated the coculture of chondrocytes and mesenchymal stem cells (MSCs) on electrospun fibrous polymer scaffolds to produce polymer/extracellular matrix (ECM) hybrid constructs with the objective of reducing the number of chondrocytes necessary to produce ample cartilage-like ECM within the scaffolds. To generate these hybrid constructs, electrospun poly(e-caprolactone) fibrous scaffolds were seeded at both high and low initial densities with five different ratios of chondrocytes to MSCs: 1:0, 1:1, 1:3, 1:5, and 0:1, and cultured for 7, 14, and 21 days."
Our news editors obtained a quote from the research from Rice University, "Glycosaminoglycan production and distribution within the three coculture groups was similar to quantities generated by chondrocyte-only controls. Conversely, as the concentration of chondrocytes was increased, the collagen content of the constructs also increased at each time point, with a 1:1 chondrocyte to MSC ratio approximating the collagen production of chondrocytes alone. Histological staining suggested that cocultured constructs mimicked the well-distributed ECM patterns of chondrocyte generated constructs, while improving greatly over the restricted distribution of matrix within MSC-only constructs. These results support the capacity of cocultures of chondrocytes and MSCs to generate cartilaginous matrix within a polymeric scaffold."
According to the news editors, the research concluded: "Further, the inclusion of MSCs in these cocultures enables the reduction of chondrocytes needed to produce cell-generated ECM."
For more information on this research see: Cell-Derived Polymer/Extracellular Matrix Composite Scaffolds for Cartilage Regeneration, Part 1: Investigation of Cocultures and Seeding Densities for Improved Extracellular Matrix Deposition. Tissue Engineering Part C-Methods, 2014;20(4):340-357. Tissue Engineering Part C-Methods can be contacted at: Mary Ann Liebert, Inc, 140 Huguenot Street, 3RD Fl, New Rochelle, NY 10801, USA (see also Biomedicine and Biomedical Engineering).
The news editors report that additional information may be obtained by contacting E.J. Levorson, Rice University, Dept. of Bioengn, Houston, TX 77005, United States. Additional authors for this research include P.M. Mountziaris, O. Hu, F.K. Kasper and A.G. Mikos.
Keywords for this news article include: Tissue Engineering, Biomedicine and Biomedical Engineering, Texas, Houston, Chondrocytes, United States, Bioengineering, Extracellular Space, Extracellular Matrix, Connective Tissue Cells, North and Central America
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