By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Transport Vesicles. According to news reporting originating from Minneapolis, Minnesota, by NewsRx correspondents, research stated, "Tunneling nanotubes (TnTs) are long, non-adherent, actin-based cellular extensions that act as conduits for transport of cellular cargo between connected cells. The mechanisms of nanotube formation and the effects of the tumor microenvironment and cellular signals on TnT formation are unknown."
Our news editors obtained a quote from the research from the University of Minnesota, "In the present study, we explored exosomes as potential mediators of TnT formation in mesothelioma and the potential relationship of lipid rafts to TnT formation. Mesothelioma cells co-cultured with exogenous mesothelioma-derived exosomes formed more TnTs than cells cultured without exosomes within 24-48 h; and this effect was most prominent in media conditions (low-serum, hyperglycemic medium) that support TnT formation (1.3-1.9-fold difference). Fluorescence and electron microscopy confirmed the purity of isolated exosomes and revealed that they localized predominantly at the base of and within TnTs, in addition to the extracellular environment. Time-lapse microscopic imaging demonstrated uptake of tumor exosomes by TnTs, which facilitated intercellular transfer of these exosomes between connected cells. Mesothelioma cells connected via TnTs were also significantly enriched for lipid rafts at nearly a 2-fold higher number compared with cells not connected by TnTs."
According to the news editors, the research concluded: "Our findings provide supportive evidence of exosomes as potential chemotactic stimuli for TnT formation, and also lipid raft formation as a potential biomarker for TnT-forming cells."
For more information on this research see: Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells. Experimental Cell Research, 2014;323(1):178-188. Experimental Cell Research can be contacted at: Elsevier Inc, 525 B Street, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Experimental Cell Research - www.elsevier.com/wps/product/cws_home/622826)
The news editors report that additional information may be obtained by contacting V. Thayanithy, University of Minnesota, Dept. of Surg, Minneapolis, MN 55455, United States. Additional authors for this research include V. Babatunde, E.L. Dickson, P. Wong, S. Oh, X. Ke, A. Barlas, S. Fujisawa, Y. Romin, A.L. Moreira, R.J. Downey, C.J. Steer, S. Subramanian, K. Manova-Todorova, M.A.S. Moore and E. Lou (see also Transport Vesicles).
Keywords for this news article include: Exosomes, Minnesota, Organelles, Minneapolis, United States, Transport Vesicles, Cytoplasmic Structures, North and Central America
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC