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Patent Issued for Low Cost Electrochemical Disposable Sensor for Measuring Glycated Hemoglobin

May 6, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- A patent by the inventors Vanjari, Siva Rama Krishna (Bangalore, IN); Bhat, Navakanta (Bangalore, IN); Srinivasan, Sampath (Bangalore, IN); Amrutur, Bharadwaj (Bangalore, IN); Kalapu, Chakrapani (Bangalore, IN); Mandal, Amit Kumar (Bangalore, IN), filed on April 18, 2012, was published online on April 22, 2014, according to news reporting originating from Alexandria, Virginia, by NewsRx correspondents (see also Indian Institute of Science).

Patent number 8702931 is assigned to Indian Institute of Science (Bangalore, IN).

The following quote was obtained by the news editors from the background information supplied by the inventors: "As is well known in the relevant arts, glycated hemoglobin (GHb) is formed in a non-enzymatic glycation pathway by hemoglobin's exposure to plasma glucose, over 120-day lifespan of the red blood cell (RBC). A buildup of glycated hemoglobin within the red cell, therefore, reflects the average level of glucose to which the cell has been exposed during its life. It is always expressed as percentage of total hemoglobin.

"Measurement of glycated hemoglobin levels may provide more accurate information on blood glucose levels over a long period of time (typically 120 days). In comparison, fasting blood glucose measurements merely indicate blood glucose levels over a much shorter period of time (typically on a daily basis). Estimation of percentage GHb may be valuable, for example, in devising optimum treatment strategies for long term management of diabetes. Various methods for the measurement of glycated hemoglobin are known in the art. Although techniques such as immunoassay, ion-exchange chromatography, electrophoresis, boronate affinity chromatography and high pressure liquid chromatography along with electrospray ionization mass spectrometry are commonly used in the laboratories, these techniques may be time consuming and may require bulky equipments. Other techniques to reduce the size of such equipments exploit the adsorption of GHb onto various materials like Zirconia nanoparticles, Aminophenyl boronic acid agarose beads, Thiophene-3-boronic acid on gold particles. However such materials may be associated with high costs, and designing these instruments may be complex. US patent no. 20100089774A1 discloses a screen printed electrode (SPE) system for detection of glycated hemoglobin and hemoglobin. However this method of detection involves elaborate use of buffers and reagents and uses potentiometric techniques to detect GHb. Addition of precise amount of liquid reagents, needs a lab technician with relevant experience and hence cannot be used by a common unskilled user. The present invention is directed towards the above problem and relates to easy to use disposable sensors for measuring percentage glycated hemoglobin in a blood sample. The inventors propose using a membrane modified with lysing agents, so that it can be used in miniaturized point of care devices for hemoglobin or glycated haemoglobin measurement. This technique eliminates the need for mixing in a separate lysis buffer which is a huge advantage in terms of making a point of care device both more user friendly and more robust. The inventors also propose the methodology for the specific detection of GHb and Hb."

In addition to the background information obtained for this patent, NewsRx journalists also obtained the inventors' summary information for this patent: "A disposable sensor for measuring glycated hemoglobin (GHb) and hemoglobin (Hb) has a GO-APBA (graphene oxide-3-Aminophenylboronic acid) coated working electrode and a GO modified working electrode. The sensor further has a reference electrode for maintaining potential and a counter electrode for maintaining charge balance and a salt-surfactant modified porous cellulose acetate membrane for lysing erythrocytes of blood cell samples and releasing hemoglobin molecules. The electrodes of the sensor are screen printed electrodes (SPE) and the lysis membrane is stitched on top of these electrodes. Detection of GHb is done using electrochemical impedance spectroscopy. A potential is applied between working electrode and reference electrode and the impedance between working electrode and counter electrode is determined. In another embodiment a two electrode system is used. The potential is applied between working and reference electrodes and the resultant current is measured between working and reference electrodes. A counter electrode is not used."

URL and more information on this patent, see: Vanjari, Siva Rama Krishna; Bhat, Navakanta; Srinivasan, Sampath; Amrutur, Bharadwaj; Kalapu, Chakrapani; Mandal, Amit Kumar. Low Cost Electrochemical Disposable Sensor for Measuring Glycated Hemoglobin. U.S. Patent Number 8702931, filed April 18, 2012, and published online on April 22, 2014. Patent URL:

Keywords for this news article include: Chemistry, Hemoglobins, Blood Proteins, Electrochemical, Indian Institute of Science.

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Source: Life Science Weekly

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