By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Nanoparticles are discussed in a new report. According to news reporting from Wuxi, People's Republic of China, by NewsRx journalists, research stated, "A highly sensitive and specific multiplex method for the simultaneous detection of three pathogenic bacteria was fabricated using multicolor upconversion nanoparticles (UCNPs) as luminescence labels coupled with aptamers as the molecular recognition elements. Multicolor UCNPs were synthesized via doping with various rare-earth ions to obtain well-separated emission peaks."
The news correspondents obtained a quote from the research from Jiangnan University, "The aptamer sequences were selected using the systematic evolution of ligands by exponential enrichment (SELEX) strategy for Staphylococcus aureus, Vibrio parahemolyticus, and Salmonella typhimurium. When applied in this method, aptamers can be used for the specific recognition of the bacteria from complex mixtures, including those found in real food matrixes. Aptamers and multicolor UCNPs were employed to selectively capture and simultaneously quantify the three target bacteria on the basis of the independent peaks. Under optimal conditions, the correlation between the concentration of three bacteria and the luminescence signal was found to be linear from 50-10(6) cfu mL(-1). Improved by the magnetic separation and concentration effect of Fe3O4 magnetic nanoparticles, the limits of detection of the developed method were found to be 25, 10, and 15 cfu mL(-1) for S. aureus, V. parahemolyticus, and S. typhimurium, respectively. The capability of the bioassay in real food samples was also investigated, and the results were consistent with experimental results obtained from plate-counting methods."
According to the news reporters, the research concluded: "This proposed method for the detection of various pathogenic bacteria based on multicolor UCNPs has great potential in the application of food safety and multiplex nanosensors."
For more information on this research see: Simultaneous Aptasensor for Multiplex Pathogenic Bacteria Detection Based on Multicolor Upconversion Nanoparticles Labels. Analytical Chemistry, 2014;86(6):3100-3107. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting S.J. Wu, Jiangnan Univ, Synerget Innovat Center Food Safety & Nutr, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, People's Republic of China. Additional authors for this research include N. Duan, Z. Shi, C.C. Fang and Z.P. Wang (see also Nanoparticles).
Keywords for this news article include: Wuxi, Asia, Nanotechnology, Emerging Technologies, People's Republic of China
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