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Data on Essential Amino Acids Detailed by Researchers at Cracow University of Technology

May 6, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Essential Amino Acids have been published. According to news reporting originating from Krakow, Poland, by NewsRx correspondents, research stated, "Phenolic acids are an important group of plant secondary metabolites with various, valuable therapeutic properties. Apart from plants growing in the open air, tissue cultures can be an alternative source of the secondary metabolites."

Our news editors obtained a quote from the research from the Cracow University of Technology, "The yield of their accumulation in in vitro cultures can be increased by different methods, including culture medium supplementation with precursors, elicitors and changing the standard amounts of the medium components. The purpose of this study was to investigate the influence of the precursor (L-phenylalanine), the elicitor (methyl jasmonate) and a higher sucrose concentration on the phenolic acids accumulation in the agitated shoot cultures of Exacum affine Balf. f. ex Regel (Gentianaceae). Qualitative and quantitative analyses of the phenolic acids in methanolic extracts from the biomass were conducted by applying the HPLC method. Fourteen phenolic acids and cinnamic acid were found in all samples. The total content of free phenolic acids increased from approximately 0.242% to 0.635% (2.6-fold) and the total content of the whole phenolic acids (free and bound) from 0.712% to 1.160% (1.6-fold)."

According to the news editors, the research concluded: "The studies show that the best variant for the accumulation of most of the identified phenolic acids contained 6% of sucrose (double the standard amount), L-phenylalanine 1.6g L-1 of medium and methyl jasmonate 100 mu M. The analysis of the results in the experiment presented here showed that it is possible to increase the accumulation of the phenolic acids in Exacum affine shoot cultures by adding the precursor (L-phenylalanine), the elicitor (methyl jasmonate) and by increasing the sucrose concentration."

For more information on this research see: The influence of L-phenylalanine, methyl jasmonate and sucrose concentration on the accumulation of phenolic acids in Exacum affine Balf. f. ex Regel shoot culture. Acta Biochimica Polonica, 2014;61(1):47-53. Acta Biochimica Polonica can be contacted at: Acta Biochimica Polonica, Pasteura 3, 02-093 Warsaw, Poland (see also Essential Amino Acids).

The news editors report that additional information may be obtained by contacting E. Skrzypczak-Pietraszek, Cracow Univ Technol, Inst Appl Informat, Krakow, Poland. Additional authors for this research include J. Slota and J. Pietraszek.

Keywords for this news article include: Krakow, Poland, Europe, Phenylalanine, Aromatic Amino Acids, Essential Amino Acids

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Source: Life Science Weekly

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