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Data from National Institute of Advanced Industrial Science and Technology (NIAIST) Provide New Insights into Microbiology

May 6, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Life Science Research have been presented. According to news originating from Hokkaido, Japan, by NewsRx correspondents, research stated, "We developed a novel protein complex isolation method using a single-chain variable fragment (scFv) based phage display library in a two-step purification procedure."

Our news journalists obtained a quote from the research from the National Institute of Advanced Industrial Science and Technology (NIAIST), "We adapted the antibody-based phage display technology which has been developed for single target proteins to a protein mixture containing about 300 proteins, mostly subunits of Thermoplasma acidophilum complexes. T. acidophilum protein specific phages were selected and corresponding scFvs were expressed in Escherichia coli. E. coil cell lysate containing the expressed His-tagged scFv specific against one antigen protein and T. acidophilum crude cell lysate containing intact target protein complexes were mixed, incubated and subjected to protein purification using affinity and size exclusion chromatography steps."

According to the news editors, the research concluded: "This method was confirmed to isolate intact particles of thermosome and proteasome suitable for electron microscopy analysis and provides a novel protein complex isolation strategy applicable to organisms where no genetic tools are available."

For more information on this research see: Protein complex purification from Thermoplasma acidophilum using a phage display library. Journal of Microbiological Methods, 2014;98():15-22. Journal of Microbiological Methods can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Microbiological Methods - www.elsevier.com/wps/product/cws_home/506034)

The news correspondents report that additional information may be obtained from A. Hubert, Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Sapporo, Hokkaido 0628517, Japan. Additional authors for this research include Y. Mitani, T. Tamura, M. Boicu and I. Nagy (see also Life Science Research).

Keywords for this news article include: Asia, Japan, Hokkaido, Life Science Research

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Source: Life Science Weekly