By a News Reporter-Staff News Editor at Science Letter -- Current study results on DNA Research have been published. According to news originating from Braga, Portugal, by NewsRx correspondents, research stated, "The present study, reports the utilization of berenil as ligand in a negative pseudo-affinity chromatographic step to purify the plasmid pVAX1-LacZ from Escherichia coli clarified lysates. The chromatographic support was prepared by coupling berenil to epoxy-activated Sepharose and was qualitatively and quantitatively characterized using scanning electron microscopy, Fourier transformed infrared spectroscopy and elemental analysis."
Our news journalists obtained a quote from the research from the University of Minho, "The clarified lysate was loaded onto the berenil-Sepharose support with 0.55 M of ammonium sulphate in the eluent, achieving the immediate elution of plasmid DNA. The impurities tightly bound to the support, were eluted after decreasing the salt concentration to 0 M. The overall process enabled the recovery of 87% of loaded plasmid DNA with a HPLC purity of >> 99% and according to FDA specifications. This method represents an alternative approach to the previous utilization of the same chromatographic pseudo-affinity support in a positive mode."
According to the news editors, the research concluded: "It uses lower amounts of salt and one-step chromatographic procedure, resulting in smaller operating time and costs and representing an alternate procedure for plasmid DNA purification."
For more information on this research see: Negative pseudo-affinity chromatography for plasmid DNA purification using berenil as ligand. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2014;944():39-42. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also DNA Research).
The news correspondents report that additional information may be obtained from C. Caramelo-Nunes, University of Minho, Center Chem, P-4710057 Braga, Portugal. Additional authors for this research include M.P. Gabriel, P. Almeida, J.C. Marcos and C.T. Tomaz.
Keywords for this news article include: Braga, Europe, Portugal, DNA Research
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