By a News Reporter-Staff News Editor at Science Letter -- Data detailed on Biological Factors have been presented. According to news originating from Zhenjiang, People's Republic of China, by NewsRx correspondents, research stated, "An amplified electrochemical impedimetric aptasensor for ochratoxin A (OTA) was developed with picomolar sensitivity. A facile route to fabricate gold nanoparticles covalently bound reduced graphene oxide (AuNPs-rGO) resulted in a large number of well-dispersed AuNPs on graphene sheets with tremendous binding sites for DNA, since the single rGO sheet and each AuNP can be loaded with hundreds of DNA strands."
Our news journalists obtained a quote from the research from Jiangsu University, "An aptasensor with sandwich model was fabricated which involved thiolated capture DNA immobilized on a gold electrode to capture the aptamer, then the sensing interface was incubated with OTA at a desired concentration, followed by AuNPs-rGO functionalized reporter DNA hybridized with the residual aptamers. By exploiting the AuNPs-rGO as an excellent signal amplified platform, a single hybridization event between aptamer and reporter DNA was translated into more than 10(7) redox events, leading to a substantial increase in charge-transfer resistance (R-ct) by 7 similar to orders of magnitude compared with that of the free aptamer modified electrode. Such designed aptasensor showed a decreased response of R-ct to the increase of OTA concentrations over a wide range of 1 pg mL(-1)-50 ng mL(-1) and could detect extremely low OTA concentration, namely, 0.3 pg mL(-1) or 0.74 pM, which was much lower than that of most other existed impedimetric aptasensors."
According to the news editors, the research concluded: "The signal amplification platform presented here would provide a promising model for the aptamer-based detection with a direct impedimetric method."
For more information on this research see: Amplified impedimetric aptasensor based on gold nanoparticles covalently bound graphene sheet for the picomolar detection of ochratoxin A. Analytica Chimica Acta, 2014;806():128-135. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
The news correspondents report that additional information may be obtained from L. Jiang, Jiangsu Univ, Sch Chem & Chem Engn, Key Lab Modern Agr Equipment & Technol, Zhenjiang 212013, People's Republic of China. Additional authors for this research include J. Qian, X.W. Yang, Y.T. Yan, Q. Liu, K. Wang and K. Wang (see also Biological Factors).
Keywords for this news article include: Asia, Zhenjiang, Mycotoxins, Ochratoxins, DNA Research, Nanotechnology, Biological Factors, Gold Nanoparticles, Emerging Technologies, People's Republic of China
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