By a News Reporter-Staff News Editor at Science Letter -- A new study on Peptides is now available. According to news reporting originating from Shanghai, People's Republic of China, by NewsRx correspondents, research stated, "Zwitterionic hydrophilic interaction chromatography (ZIC-HILIC) materials have been increasingly attractive in glycopeptide enrichment. However, the traditional ZIC-HILIC materials are modified with monolayer zwitterionic molecules on the surface, therefore, the hydrophilicity, detection sensitivity and loading capacity are limited."
Our news editors obtained a quote from the research from the East China University of Science and Technology, "In this work, we synthesized novel silica nanoparticles with uniform poly(2( methacryloyloxy) ethyl) dimethyl-(3-sul-fopropyl) ammonium hydroxide (PMSA) brushes grafted onto the surface via reversible addition-fragmentation chain transfer (RAFT) polymerization (denoted as SiO2-RAFT@ PMSA). The resulting SiO2-RAFT@ PMSA nanoparticles demonstrated low detection limit (10 fmol) and high recovery yield (over 88%) for glycopeptide enrichment from tryptic digest of human IgG. The SiO2-RAFT@ PMSA nanoparticles were further applied for the analysis of mouse liver glycoproteome, a total number of 303 unique N-glycosylation sites corresponding to 185 glycoproteins was reliably profiled in three replicate nano-LC-MS/MS runs. Significantly, more glycopeptides were identified than those of nanoparticles, monolayer MSA molecules modified SiO2@single-MSA and nonuniform multilayer PMSA brushes coated SiO2@PMSA, as well as commercial ZIC@HILIC beads and Click Maltose beads. The excellent performance of SiO2-RAFT@PMSA nanoparticles results from the non-fouling property, a large quantity of functional molecules and suitable link arms provided by uniform PMSA brushes, as well as efficient interaction between glycopeptides and uniform PMSA brushes. It is concluded that the synthesized SiO2-RAFT@PMSA nanoparticles exhibit great potential in glycoproteome analysis."
According to the news editors, the research concluded: "Moreover, this strategy to modify nanopaticles with uniform polymer brushes via RAFT polymerization can also be explored to design other types of materials for bioseparation application."
For more information on this research see: Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides. Analytica Chimica Acta, 2014;809():61-68. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
The news editors report that additional information may be obtained by contacting G. Huang, E. China University of Science & Technology, Shanghai Key Lab Funct Mat Chem, Shanghai 20023, People's Republic of China. Additional authors for this research include Z.C. Xiong, H.Q. Qin, J. Zhu, Z. Sun, Y. Zhang, X.J. Peng, J.J. Ou and H.F. Zou (see also Peptides).
Keywords for this news article include: Asia, Shanghai, Nanoparticle, Glycopeptides, Nanotechnology, Emerging Technologies, People's Republic of China
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