By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on DNA Research are presented in a new report. According to news originating from Shandong, People's Republic of China, by NewsRx correspondents, research stated, "Herein, we developed a new electrochemical immunoassay for detecting of DNA methylation, analyzing DNA methyltransferase (MTase) activity and evaluating the inhibition effects of pesticides on DNA MTase. The detection strategy was based on the advantages of methyl binding domain protein (MBD1) on specifically recognizing symmetrical methylated cytosine, immune recognition reaction between antibody and antigen, and antibody functionalized gold nanoparticles (AuNPs) with signal amplification effects."
Our news journalists obtained a quote from the research from Shandong Agricultural University, "For improving the detection sensitivity, dual signal amplification was employed with the first of anti-his-tag antibody tagged AuNPs and the second of alkaline phosphatase (ALP) labeled IgG tagged AuNPs. The ALP converted 1-naphthyl phosphate into 1-naphthol and the electrochemical signal of 1-naphthyl showed a wider linear relationship of 0.5-500 pM with methylated DNA and 0.03-50 unit/mL with M. SssI concentration. The detection limits were 0.17 pM and 0.01 unit/mL, respectively. In addition, we also investigated the inhibition effects of chlorpyrifos and methyl parathion on MTase activity, which might provide useful information on carcinogenic mechanism of pesticide."
According to the news editors, the research concluded: "This research also verified that the developed method could be applied to screen the inhibitors of DNA MTase and develop new anticancer drugs."
For more information on this research see: Electrochemical immunosensor for DNA methyltransferase activity assay based on methyl CpG-binding protein and dual gold nanoparticle conjugate-based signal amplification. Sensors and Actuators B-Chemical, 2014;192():143-149. Sensors and Actuators B-Chemical can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also DNA Research).
The news correspondents report that additional information may be obtained from Z.N. Xu, Shandong Agriculture University, Coll Chem & Mat Sci, Tai An 271018, Shandong, People's Republic of China. Additional authors for this research include H.S. Yin, L.L. Huo, Y.L. Zhou and S.Y. Ai.
Keywords for this news article include: Asia, Antibodies, Shandong, Chemistry, Immunology, DNA Research, Blood Proteins, Nanotechnology, Electrochemical, Immunoglobulins, Gold Nanoparticles, Methyltransferases, Emerging Technologies, People's Republic of China, One-Carbon Group Transferases
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