By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Researchers detail new data in Biotechnology. According to news reporting from Vancouver, Canada, by NewsRx journalists, research stated, "With recent advancements in stem cell-based gene therapy, concerns about safety have grown. Stem cell-based gene therapies may pose the risk of immunological problems and oncogenesis."
The news correspondents obtained a quote from the research from the University of British Columbia, "We investigated the feasibility of treating glioblastomas with neural stem cells [(NSCs), HB1.F3 cells] expressing double prodrug enzymes [cytosine deaminase (CD) and tyrosine kinase (TK)] to eliminate the NSCs following treatment for safety purposes. First, the in vitro and in vivo therapeutic efficacies of NSCs engineered with double prodrug enzymes (HB1.F3-CD.TK cells) were compared to cells expressing a single prodrug enzyme (HB1.F3-CD). Second, the degree of safety achieved by NSC elimination was compared with an in vitro viability assay of the NSCs after treatment with the double prodrugs. We further compared the differences in in vivo proliferation of control, single prodrug enzyme and double prodrug enzyme expressing NSCs. HB1.F3-CD.TK cells showed a better or comparable treatment outcome than HB1.F3-CD cells in vitro and in vivo. For safety, HB1.F3-CD.TK cells showed the least viability in vitro after treatment with prodrugs compared to HB1.F3 and HB1.F3-CD cells. Additionally, the in vivo proliferation among the injected NSCs found in the tumor was the smallest for HB1.F3-CD.TK cells."
According to the news reporters, the research concluded: "Double-prodrug enzyme-directed gene therapy shows good therapeutic efficacy as well as efficient eradication of the NSCs to ensure safety for clinical applications of stem cell-based gene therapies."
For more information on this research see: Double suicide gene therapy using human neural stem cells against glioblastoma: double safety measures. Journal of Neuro-Oncology, 2014;116(1):49-57. Journal of Neuro-Oncology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Journal of Neuro-Oncology - www.springerlink.com/content/0167-594x/)
Our news journalists report that additional information may be obtained by contacting J.Y. Lee, University of British Columbia, Dept. of Med, UBC Hosp, Div Neurol, Vancouver, BC, Canada. Additional authors for this research include D.H. Lee, H.A. Kim, S.A. Choi, H.J. Lee, C.K. Park, J.H. Phi, K.C. Wang, S.U. Kim and S.K. Kim (see also Biotechnology).
Keywords for this news article include: Biotechnology, Canada, Oncology, Vancouver, Treatment, Gene Therapy, Glioblastoma, Bioengineering, British Columbia, Stem Cell Research, Enzymes and Coenzymes, North and Central America
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